碳量子点的荧光性质及其在药物分析中的应用

发布时间：2018-05-24 08:54

本文选题：微波合成法 + 碳量子点　；参考：《郑州大学》2014年硕士论文

【摘要】：碳量子点作为新兴的荧光纳米材料，以其优良的光学性能和低毒性逐步发展为一种新型的荧光探针。目前，碳量子点引起各国研究者的关注，已被广泛应用于环境检测、活体成像、生物探针以及光电器件等诸多领域。碳量子点的合成方法已日趋成熟，但对碳量子点的荧光性能的影响因素研究报道较少。其中，利用量子点的特性作为荧光探针分析药物小分子、核酸、蛋白等生物大分子含量的报道已有一些，但是利用碳量子点作为荧光探针来进行药物分析的文献却并不多见。本论文试图对碳量子点的荧光性质影响因素进行探究；寻找新的荧光探针，建立灵敏便捷的药物小分子定量检测方法。以荧光猝灭法为主要的研究方法，利用紫外-可见吸收光谱、氯化钠离子分析实验以及荧光猝灭程度与温度的关系等实验方法对碳量子点与药物的结合机理进行了探讨。全文主要内容包括如下几个部分： 1.实验采用简单快速的微波加热法合成了水溶性碳量子点，并利用透射电镜，紫外可见吸收光谱，荧光光谱，红外光谱及纳米粒度仪对其形态学，光学及表面化学性质进行了表征；对各种影响因素如表面活性剂、氧化剂、还原剂、常见离子、荧光染料、pH、温度及碳纳米材料等对碳量子点的荧光性质的影响，进行初步研究和分析。此外，初步探究了四环素类和黄酮类部分药物与碳量子点的相互作用，为该类药物的分析提供一种检测方法。 2.基于姜黄素对微波加热法合成的水溶性碳量子点的荧光猝灭作用，将碳量子点作为荧光探针。研究发现姜黄素的加入能够使碳量子点的荧光信号显著降低。在pH4.0的Britton-Robinson的缓冲溶液中，姜黄素的浓度与碳量子点的荧光强度变化成线性关系，由此建立了一种姜黄素的定量方法。其中姜黄素的线性范围在0.03-3.0μg mL-1，相关系数r=0.9984，检测限为4.42×10-10g mL-1，将此法用于合成样品及实际样品分析，结果令人满意。利用紫外可见吸收光谱，氯化钠离子实验和荧光猝灭程度与温度的关系对姜黄素与碳量子点相互作用的可能机理进行了探讨。 3.探究了盐酸四环素（TC）对水溶性碳量子点的荧光猝灭作用。实验采用碳量子点作为荧光探针，研究发现盐酸四环素能够使碳量子点的荧光信号降低。在pH4.0的Britton-Robinson的缓冲溶液中，盐酸四环素的浓度与碳量子点的荧光强度变化成线性关系，由此建立了盐酸四环素的定量方法。在优化的实验条件下，，盐酸四环素的线性范围在0.3-30μg mL-1，相关系数r=0.9991，检测限为2.83×10-9g mL-1，并将此法用于合成样品及实际样品分析，结果令人满意。利用紫外可见吸收光谱，氯化钠离子实验和荧光猝灭程度与温度的关系对盐酸四环素与碳量子点相互作用的可能机理进行了探讨。
[Abstract]:Carbon quantum dots (QDs), as new fluorescent nanomaterials, have been developed as novel fluorescent probes with their excellent optical properties and low toxicity. At present, carbon quantum dots (QDs) have attracted the attention of researchers all over the world, and have been widely used in many fields, such as environmental detection, living imaging, biological probes and optoelectronic devices. The synthesis methods of carbon quantum dots have become more and more mature, but there are few reports on the factors influencing the fluorescence properties of carbon quantum dots. Among them, there have been some reports on the use of quantum dots as fluorescence probes for the analysis of drug micromolecules, nucleic acids, proteins and other biological macromolecules, but there are few literatures on the use of carbon quantum dots as fluorescent probes for drug analysis. In this paper, we try to explore the factors affecting the fluorescence properties of carbon quantum dots, find new fluorescent probes, and establish a sensitive and convenient method for quantitative detection of small molecules of drugs. The binding mechanism of carbon quantum dots (QDs) with drugs was investigated by UV-Vis absorption spectra, sodium chloride ion analysis and the relationship between fluorescence quenching degree and temperature. The main contents of this paper are as follows: 1. The water-soluble carbon quantum dots were synthesized by a simple and rapid microwave heating method. The morphology of the QDs was characterized by transmission electron microscope, UV-Vis absorption spectrum, fluorescence spectrum, infrared spectrum and nano-particle size analyzer. The optical and surface chemical properties were characterized, and the effects of various factors, such as surfactant, oxidant, reductant, common ions, pH of fluorescent dye, temperature and carbon nanomaterials, on the fluorescence properties of carbon quantum dots were studied. To carry out preliminary research and analysis. In addition, the interaction of tetracycline and flavonoids with carbon quantum dots (QDs) was preliminarily investigated, which provided a method for the analysis of tetracycline and flavonoids. 2. Based on the fluorescence quenching effect of curcumin on water-soluble carbon quantum dots synthesized by microwave heating, carbon quantum dots were used as fluorescence probes. It is found that curcumin can significantly reduce the fluorescence signal of carbon quantum dots. In the buffer solution of Britton-Robinson of pH4.0, the concentration of curcumin was linearly correlated with the fluorescence intensity of carbon quantum dots, and a quantitative method of curcumin was established. The linear range of curcumin was 0.03-3.0 渭 g mL ~ (-1), the correlation coefficient was 0.9984, and the detection limit was 4.42 脳 10 ~ (-10) g mL ~ (-1). The method was applied to the analysis of synthetic and actual samples with satisfactory results. The possible mechanism of the interaction between curcumin and carbon quantum dots was investigated by UV-Vis absorption spectra, sodium chloride ion experiments and the relationship between fluorescence quenching degree and temperature. 3. The fluorescence quenching effect of tetracycline hydrochloride (TC) on water soluble carbon quantum dots (QDs) was investigated. Using carbon quantum dots as fluorescence probe, it is found that tetracycline hydrochloride can reduce the fluorescence signal of carbon quantum dots. In the buffer solution of Britton-Robinson of pH4.0, the concentration of tetracycline hydrochloride was linearly correlated with the fluorescence intensity of carbon quantum dots. A quantitative method for tetracycline hydrochloride was established. Under the optimized experimental conditions, the linear range of tetracycline hydrochloride was 0.3-30 渭 g mL ~ (-1), the correlation coefficient was 0.9991, and the detection limit was 2.83 脳 10 ~ (-9) g mL ~ (-1). The method was applied to the analysis of synthetic and practical samples with satisfactory results. The possible mechanism of the interaction between tetracycline hydrochloride and carbon quantum dots was investigated by UV-Vis absorption spectra, sodium chloride ion experiments and the relationship between fluorescence quenching degree and temperature.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R917

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