二氢杨梅素对小鼠葡聚糖硫酸钠结肠炎的干预作用

发布时间：2018-05-28 01:41

本文选题：二氢杨梅素 + 结肠炎　；参考：《中国新药与临床杂志》2017年05期

【摘要】：目的探讨二氢杨梅素(DMY)对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎(UC)的作用效果。方法将60只Balb/C小鼠随机分为正常对照组、模型组、DMY低剂量组(50 mg·kg~(-1)·d~(-1))、DMY中剂量组(100 mg·kg~(-1)·d~(-1))、DMY高剂量组(200 mg·kg~(-1)·d~(-1))和5-氨基水杨酸组(50 mg·kg~(-1)·d~(-1)),各10只。正常对照组小鼠每日给予蒸馏水饮用,其余组小鼠给予4%DSS连续饮用7 d,诱导小鼠急性UC模型,与此同时给予对应剂量的药物灌胃治疗。7 d后处死小鼠并计算小鼠疾病活动指数(DAI)、光镜下结肠组织损伤评分(TDI),流式细胞法测定外周血中调节性T淋巴细胞(Treg)、辅助性T细胞17(Th17)占CD4+T细胞的百分比,Western blot及免疫组化法测定结肠组织中基质金属蛋白酶9(MMP 9)的表达水平。结果与正常对照组相比,模型组DAI、TDI明显升高(P0.01),Treg/Th17比值明显降低(P0.01),MMP 9表达升高(P0.05)。与模型组相比,DMY低、中、高剂量组小鼠Treg/Th17比值明显升高(P0.01),MMP 9表达降低(P0.05),DMY中、高剂量组小鼠DAI、TDI明显降低(P0.01),且随着治疗剂量增加,升高或降低幅度愈明显。结论 DMY可以显著缓解DSS诱导的小鼠UC炎症,且与剂量呈正相关。
[Abstract]:Objective to investigate the effect of dihydromyricetin (DMY) on UC induced by dextran sodium sulfate (DSS) in mice with ulcerative colitis. Methods Sixty Balb/C mice were randomly divided into normal control group, model group with low dose of DMY (50 mg / kg / kg) / d ~ (-1), medium dose group (n = 10) and control group (n = 10), respectively, with a dose of 100 mg / d ~ (-1) / d ~ (-1) (n = 10) and a group of 5 ~ (-) aminosalicylic acid (n = 10), respectively, with a dose of 200 mg / kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) d ~ (-1) ~ (-1) ~ (-1) (n = 10) and a group of 5 ~ (-) aminosalicylic acid group (n = 10). The mice in the control group were given distilled water daily and the other mice were given 4%DSS for 7 days to induce the acute UC model. At the same time, the mice were sacrificed after oral administration of the corresponding dose of drugs for 7 days, and the disease activity index (DAI) was calculated. The colonic tissue injury score (TDI) was evaluated under light microscope. The Tregn of regulatory T lymphocytes in peripheral blood was measured by flow cytometry. The expression of matrix metalloproteinase (9(MMP 9) in colon tissue was detected by Western blot and immunohistochemical method. Results compared with the normal control group, the TDI of the model group was significantly higher than that of the control group, and the ratio of Treg-Th17 to Treg-17 in the model group was significantly lower than that in the control group. The expression of MMP9 in the model group was significantly lower than that in the control group (P 0.05). Compared with the model group, the Treg/Th17 ratio in the high dose group was significantly higher than that in the model group, and the expression of MMP9 in the high dose group was significantly higher than that in the model group. In the high dose group, the expression of Treg/Th17 in the high dose group was significantly lower than that in the model group, and with the increase of the therapeutic dose, the increase or decrease was more obvious. Conclusion DMY can significantly relieve UC inflammation induced by DSS in mice and has a positive correlation with the dose.
【作者单位】：兰州大学第二医院消化科;
【基金】：国家自然科学基金(81272661) 甘肃省自然科学基金(1506RJZA204)
【分类号】：R965

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