超高效液相色谱质谱法同时测定人血浆中8种抗组胺类药物

发布时间：2018-06-08 15:11

  本文选题：抗组胺类药物 + 血药浓度　； 参考：《中国临床药理学杂志》2017年10期

【摘要】：目的建立同时测定人血浆中苯海拉明、氯苯那敏、异丙嗪、氯雷他定、非索非那定、地氯雷他定、左西替利嗪、赛庚啶浓度的超高效液相色谱质谱(UPLC-MS/MS)法。方法以盐酸吡格列酮为内标,血浆经甲醇直接沉淀后进样分析,用UPLC-MS/MS法进行检测。色谱柱为ACQUITY UPLC HSS C18柱,流动相为含0.01%甲酸的水溶液-0.01%甲酸的甲醇溶液,流速为0.3 m L·min 230001,China,电喷雾离子源,用多反应监测,正离子分段扫描分析。结果苯海拉明、氯苯那敏、异丙嗪、氯雷他定、非索非那定、地氯雷他定、左西替利嗪、赛庚啶血药浓度分别在1.15×10~(-3)~2.30×10~(-1),2.48×10~(-4)~2.48×10~(-2),2.86×10~(-3)~2.86×10~(-1),2.50×10~(-4)~2.50×10~(-2),5.50×10~(-3)~2.20,2.20×10~(-4)~2.20×10~(-2),5.70×10~(-3)~2.28,3.50×10~(-3)~3.50×10~(-1)μg·m L~(-1)线性关系良好;最低定量下限分别为1.15×10~(-3),2.48×10~(-4),2.86×10~(-3),2.50×10~(-4),5.50×10~(-3),2.20×10~(-4),5.70×10~(-3),3.50×10~(-3)μg·m L~(-1)。日内、日间精密度RSD均15%;提取回收率均65%,RSD均15%。结论该方法灵敏、快速、准确、专属性强,可用于上述8种抗组胺类药物的血药浓度监测及药代动力学研究。
[Abstract]:Objective to establish a method for the simultaneous determination of phenhydramine, chlorphenamine, promethazine, loratadine, fexofenadine, desloratadine, levcetirizine and cyproheptadine in human plasma by UPLC-MS / MS. Methods pioglitazone hydrochloride was used as internal standard, plasma was analyzed by direct precipitation of methanol, and detected by UPLC-MS / MS method. The chromatographic column was ACQUITY UPLC HSS C18 column, the mobile phase was aqueous solution containing 0.01% formic acid -0.01% formic acid methanol solution, the flow rate was 0.3 mL / min 230001 / 1 China, the electrospray ion source was monitored by multi-reaction, and the positive ion was analyzed by segmental scanning. 缁撴灉鑻�娴锋媺鏄�,姘�鑻�閭ｆ晱,寮備笝鍡，

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