没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型的作用

发布时间：2018-06-14 09:50

本文选题：慢性鼻-鼻窦炎 + 没食子酸　；参考：《中国药科大学学报》2015年05期

【摘要】：探讨没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型(CRS)的作用。收集难治性CRS患者鼻黏膜样本,分离铜绿假单胞杆菌(Pseudomonas aeruginosa)构建小鼠CRS模型,没食子酸(GA)与环丙沙星(CIP)单独或联合灌胃治疗,HE染色观察小鼠鼻黏膜病理变化,ELISA检测血清炎性因子TNF-α、IL-6、IL-8的表达、试剂盒检测SOD活性、MDA及ROS含量,Western blot检测鼻黏膜组织中TNF-α、IL-6、IL-8、IκB及NF-κB p65的表达。结果显示,GA与CIP可显著降低CRS小鼠TNF-α、IL-6、IL-8、MDA、ROS及NF-κB p65的含量,增加SOD活性及IκB的表达,且联合用药效果更显著。结果说明,GA与CIP联用治疗CRS效果优于单独用药,可能通过抑制NF-κB信号通路,下调TNF-α、IL-6、IL-8表达发挥作用。
[Abstract]:To investigate the effect of Gallic acid combined with ciprofloxacin on chronic rhinosinusitis in mice. A mouse CRS model was established by collecting nasal mucosa samples from refractory CRS patients and isolating Pseudomonas aeruginosa. The expression of serum inflammatory factor TNF- 伪, IL-6 and IL-8 was detected by Elisa in the nasal mucosa of mice treated with HE alone or in combination with ciprofloxacin (CIP), Gallic acid (GAG) and ciprofloxacin (ciprofloxacin). The activity of SOD and the content of Ros in nasal mucosa were detected by Western blot. The expression of TNF- 伪, IL-6, IL-8, I 魏 B and NF- 魏 B p65 in nasal mucosa was detected. The results showed that GA and CIP could significantly decrease the contents of TNF- 伪 IL-6, IL-8, MDAROS and NF- 魏 B p65, increase the activity of SOD and the expression of I 魏 B in CRS mice. The results showed that the combination of TNF- 伪 and CIP in the treatment of CRS was better than that in the treatment of CRS alone, which might play a role by inhibiting the signal pathway of NF- 魏 B and down-regulating the expression of TNF- 伪 -IL-6 / IL-8.
【作者单位】：沈阳医学院附属第二医院耳鼻喉科;
【分类号】：R965

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