2种粒径PLGA纳米粒细胞摄取动力学的比较

发布时间：2018-06-17 00:07

本文选题：PLGA纳米粒 + 细胞摄取动力学　；参考：《中国新药杂志》2017年06期

【摘要】：目的:比较包载有荧光香豆素-6(coumarin-6,C6)的2种粒径聚乳酸-羟基乙酸共聚物(plylactic acid-glycolic acid copolymer,PLGA)纳米粒,经肿瘤细胞的摄取动力学的差异。方法:以生物可降解材料PLGA为载体,采用乳化溶剂挥发法制备2种不同粒径的载有C6的PLGA纳米粒,以黑色素肿瘤细胞(B16)和脑胶质瘤细胞(U87)为细胞模型,分别与2种粒径的纳米粒孵育0.5,1,4,6和17 h,用荧光光度计检测细胞裂解液中C6的荧光值,计算得出2种细胞摄入的载药PLGA纳米粒的量(以C6的量表示),绘制入胞动力学曲线。应用Graph Pad Prism程序处理2种细胞得出的数据,并应用T检验法分析各组数据之间的差异。结果:孵育0.5 h后,2种粒径纳米粒在2种细胞中的摄取量均表现出显著差异;且随着时间延长,该差异加大。细胞摄取时间在6~17 h时,粒径约为150 nm的PLGA纳米粒的入胞速率明显变缓。结论:在相同的给药浓度下,小粒径纳米粒入胞速率要快于较大粒径的纳米粒。大粒径的纳米粒比小粒径纳米粒的入胞量到达平衡快,且总的入胞量要少。
[Abstract]:Aim: to compare the uptake kinetics of two kinds of polylactic acid-glycolic acid copolymery PLGA nanoparticles containing fluorescent coumarin-6 (C6). Methods: using biodegradable material PLGA as carrier, two kinds of PLGA nanoparticles containing C6 were prepared by emulsifying solvent volatilization method. Melanoma cell line B16) and glioma cell line U87) were used as cell models. The fluorescence values of C6 in the cell lysate were detected by fluorescence photometer after incubating with two kinds of nanoparticles of different diameters for 0. 5 and 17 h, respectively. The amount of PLGA nanoparticles absorbed by the two kinds of cells was calculated (expressed as the amount of C6), and the kinetic curve of cell entry was plotted. The graph Pad Prism program was used to process the data from two kinds of cells, and T test was used to analyze the difference between the two groups. Results: after incubation for 0.5 h, there were significant differences in the uptake of the two kinds of nanoparticles in the two kinds of cells, and the difference increased with the prolongation of time. When the cell uptake time was 6 ~ 17 h, the entry rate of PLGA nanoparticles with a diameter of about 150 nm slowed down obviously. Conclusion: at the same concentration, the entry rate of small nanoparticles is faster than that of larger nanoparticles. The entry amount of the large size nanoparticles is faster than that of the small size nanoparticles, and the total number of the particles is less.
【作者单位】：济宁医学院药学院;贵州大学药学院;
【基金】：山东省高等学校科技计划资助项目(J15LM65) 济宁医学院科研计划重点项目(JY2013KJ009)
【分类号】：R96;R943

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