海洋来源真菌Aspergillus sp.SCSIO 41501中含氮化合物

发布时间：2018-06-17 19:14

  本文选题：海洋真菌 + Aspergillus　； 参考：《天然产物研究与开发》2017年07期

【摘要】：通过多种色谱柱层析(包括凝胶Sephadex LH-20、正相硅胶、中压液相色谱和高效液相色谱)分离手段,从来源于南海柳珊瑚Melitodessquamata的一株共附生真菌Aspergillus sp.SCSIO 41501的发酵液中分离纯化了11个含氮化合物,它们的化学结构通过核磁共振和质谱等技术分别鉴定为13-(S)-赭曲霉素A(1)、13-(S)-甲酯化赭曲霉素A(2)、13-(R)-赭曲霉素A(3)、4-(R)-OH-赭曲霉素A(4)、18-OH-赭曲霉素A(5)、transtorine(6)、quinolactacin C1(7)、marinamide(8)、methyl marinamide(9)、aspergilliamide(10)、anoectochine(11)。所有化合物进行了抗菌实验,其中化合物2对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌具有较强的活性,其MIC值分别为31.25、7.8、15.6μg/m L,化合物3显示中等抗菌活性,其MIC值分别为62.5、31.25、62.5μg/m L。
[Abstract]:It was separated by a variety of chromatographic column chromatography (including gel Sephadex LH-20, silica gel, medium pressure liquid chromatography and high performance liquid chromatography). Eleven nitrogen-containing compounds were isolated and purified from the fermentation broth of a co-epiphytic fungus Aspergillus sp. SCSIO 41501 from Melitodessquamata in the South China Sea. Their chemical structures were identified by NMR and MS techniques as 13 Szapin, ochratoxin A, 13 -S ~ (1) -Methylated Aspergillus aspergilli (AJ), 13 ~ (13) -Rn-OH-1, 4 ~ (4) R ~ (-) -OH -ochratin A _ (418-OH) -OHochisin A _ (418-OH) -OHZ, A _ (418-OH) -Ochratin A _ (5) Toxolactacin in C _ (1) ~ (7) a marinamelintosine _ (8) marmarinamide9e (a) aspergillium spp. (10) a kind of anectochytochinec (1), a kind of anectochinetochlor, a kind of aspergillea spp. All the compounds were tested for antimicrobial activity. Compound 2 had strong activity against Escherichia coli, Bacillus subtilis and Staphylococcus aureus with MIC values of 31.257.8U 15.6 渭 g / mL and 62.5 渭 g / mL for compound 3. The MIC values of compound 3 were 62.5 渭 g / mL and 31.25 渭 g / mL, respectively.
【作者单位】： 中国科学院南海海洋研究所中国科学院海洋生物资源可持续利用重点实验室;中国科学院大学;
【基金】：国家自然科学基金(41376160;81673326)
【分类号】：R915
，

本文编号：2032156