环丙沙星体外诱导铜绿假单胞菌对群体感应系统正常与缺陷最低抑菌浓度的影响
发布时间：2018-08-21 09:43
【摘要】：目的:探究群体密度(QS)系统正常与缺陷的铜绿假单胞菌(PA)通过环丙沙星诱导后对其最低抑菌浓度(MIC)影响的区别。方法:将PA按照前期研究的基因测序方法分成正常组和缺陷组。采用琼脂倍比稀释法测定对环丙沙星的MIC值,用1/2MIC、1MIC、2MIC、4MIC的环丙沙星各诱导5代,保存1代、3代和5代的菌株。诱导浓度和诱导代数对MIC值的影响采用重复测量方差分析方法,正常组与缺陷组的比较采用秩和检验。结果:两组组内比较:在同一浓度下,2组各随着诱导代数增加MIC值均增加(P均0.05);在同一代数下随着诱导浓度的增加,正常组第3代和第5代的MIC值也增加(P均0.05),缺陷组第3代和第5代的MIC值差异有统计学意义(P均0.05)。2组组间比较:QS系统分组与诱导代数无交互效应(P0.05),且缺陷菌株MIC值都低于正常菌株;与诱导浓度存在交互效应(P0.05)。固定代数固定浓度两组MIC值比较:在1MIC诱导的第3代和第5代,2组之间有统计学差异(P0.05)。结论:不同诱导浓度对QS系统正常组和缺陷组MIC值的影响有区别。
[Abstract]:Aim: to investigate the difference between normal and defective Pseudomonas aeruginosa (PA) induced by ciprofloxacin (ciprofloxacin) on its minimum inhibitory concentration (MIC). Methods: PA was divided into normal group and defective group according to the previous gene sequencing method. The MIC value of ciprofloxacin was determined by Agar doubling dilution method. The ciprofloxacin of 1 / 2MIC1 MIC2MIC2MIC4MIC was induced for 5 generations each, and the strains of the third and fifth generations were preserved in the first generation of ciprofloxacin. The influence of induced concentration and induction algebra on MIC value was analyzed by repeated measurement variance, and rank sum test was used to compare normal group with defect group. Results: the MIC values of each group increased with the increase of induction algebra at the same concentration (P < 0. 05), and increased with the increase of induced concentration under the same algebra. The MIC values of the third and fifth generations of the normal group were also increased (P 0.05), and the MIC values of the third and fifth generation of the defect group were significantly different (P 0.05). 2 there was no interaction between the group of groups with the induced algebra and the group of groups (P0.05), and the MIC of the defective strain of the group was higher than that of the control group (P0.05). The values were lower than those of normal strains. There was an interaction effect with the induced concentration (P0.05). Comparison of MIC values between the two groups of fixed algebraic concentration: there was statistical difference between the third generation and the fifth generation group induced by 1MIC (P0.05). Conclusion: the effects of different inductive concentrations on the MIC values of normal and defective QS systems are different.
【作者单位】： 山西医科大学药学院;山西医科大学第二医院药学部;
【基金】：山西省卫生计生委科技攻关项目(编号:2014045)
【分类号】：R96
[Abstract]:Aim: to investigate the difference between normal and defective Pseudomonas aeruginosa (PA) induced by ciprofloxacin (ciprofloxacin) on its minimum inhibitory concentration (MIC). Methods: PA was divided into normal group and defective group according to the previous gene sequencing method. The MIC value of ciprofloxacin was determined by Agar doubling dilution method. The ciprofloxacin of 1 / 2MIC1 MIC2MIC2MIC4MIC was induced for 5 generations each, and the strains of the third and fifth generations were preserved in the first generation of ciprofloxacin. The influence of induced concentration and induction algebra on MIC value was analyzed by repeated measurement variance, and rank sum test was used to compare normal group with defect group. Results: the MIC values of each group increased with the increase of induction algebra at the same concentration (P < 0. 05), and increased with the increase of induced concentration under the same algebra. The MIC values of the third and fifth generations of the normal group were also increased (P 0.05), and the MIC values of the third and fifth generation of the defect group were significantly different (P 0.05). 2 there was no interaction between the group of groups with the induced algebra and the group of groups (P0.05), and the MIC of the defective strain of the group was higher than that of the control group (P0.05). The values were lower than those of normal strains. There was an interaction effect with the induced concentration (P0.05). Comparison of MIC values between the two groups of fixed algebraic concentration: there was statistical difference between the third generation and the fifth generation group induced by 1MIC (P0.05). Conclusion: the effects of different inductive concentrations on the MIC values of normal and defective QS systems are different.
【作者单位】： 山西医科大学药学院;山西医科大学第二医院药学部;
【基金】：山西省卫生计生委科技攻关项目(编号:2014045)
【分类号】：R96
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