红树林、海洋沉积物及北极3种不同生境真菌的分离及代谢产物活性筛选
发布时间：2018-10-08 11:02
【摘要】：目的比较红树林、海洋沉积物及北极3种不同生境中可培养真菌的物种多样性、代谢产物多样性及抗菌活性,从中分离、筛选活性代谢产物产生菌。方法采用稀释涂布平板法分离所采集样品中的真菌,根据形态学特征进行菌株排重,利用抗菌活性模型筛选菌株,利用高效液相色谱检测技术研究其代谢产物多样性。结果从3种不同生境的28份样品中共分离到真菌192株,其中68株具有抗菌活性,12株具有一系列类似特征吸收峰。结论红树林生境中可培养真菌资源丰富,代谢产物活性及多样性也比另外两种生境丰富,揭示了不同生境中可培养真菌的生态分布特点,为特殊生境真菌资源的开发利用提供了背景资料。
[Abstract]:Objective to compare the species diversity, metabolite diversity and antimicrobial activity of culturable fungi in mangrove, marine sediment and Arctic habitats. Methods the fungi were isolated from the collected samples by the dilution coating plate method. The strain weight was excreted according to the morphological characteristics. The strain was screened by the antibacterial activity model and the diversity of the metabolites was studied by HPLC. Results 192 strains of fungi were isolated from 28 samples from 3 different habitats, of which 68 strains had antimicrobial activity and 12 strains had a series of similar absorption peaks. Conclusion the culturable fungi resources in mangrove habitats are abundant, and the activity and diversity of metabolites are more abundant than those of the other two habitats, which reveals the ecological distribution characteristics of culturable fungi in different habitats. Background data are provided for the exploitation and utilization of fungal resources in special habitats.
【作者单位】： 武汉大学药学院组合生物合成与新药发现教育部重点实验室;中国典型培养物保藏中心;热带生物技术研究所;
【基金】：欧盟第七框架项目(No.312184)
【分类号】：R915
本文编号：2256507
[Abstract]:Objective to compare the species diversity, metabolite diversity and antimicrobial activity of culturable fungi in mangrove, marine sediment and Arctic habitats. Methods the fungi were isolated from the collected samples by the dilution coating plate method. The strain weight was excreted according to the morphological characteristics. The strain was screened by the antibacterial activity model and the diversity of the metabolites was studied by HPLC. Results 192 strains of fungi were isolated from 28 samples from 3 different habitats, of which 68 strains had antimicrobial activity and 12 strains had a series of similar absorption peaks. Conclusion the culturable fungi resources in mangrove habitats are abundant, and the activity and diversity of metabolites are more abundant than those of the other two habitats, which reveals the ecological distribution characteristics of culturable fungi in different habitats. Background data are provided for the exploitation and utilization of fungal resources in special habitats.
【作者单位】： 武汉大学药学院组合生物合成与新药发现教育部重点实验室;中国典型培养物保藏中心;热带生物技术研究所;
【基金】：欧盟第七框架项目(No.312184)
【分类号】：R915
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