体内药物分析的HPLC柱切换平台搭建与研究进展

发布时间：2018-10-10 19:36

【摘要】：目的:为医院药学部门搭建高效液相色谱柱切换平台,简化复杂样品的处理过程提供参考。方法:以"Column switching""柱切换"等为关键词,检索Elsevier、Springer、中国知网等数据库,查阅1970-2014年国内外涉及临床药物分析柱切换的相关文献,以柱切换色谱网络的搭建为主线,对不同的传递技术、一级柱和柱切换出现的问题进行归纳和总结。结果与结论:色谱网络的搭建包括第一级色谱柱,直接、间接和反向传输形式及检测器的选择。离线去蛋白、中心切割因较好的耐久度、经济性和去杂质能力,适合医院大通量样本的日常检测。直接进样、在线去蛋白与反冲传递技术的组合已成为目前发展趋势,随着通道限制性填料的成熟,此种组合应用将会更加广泛。具体选择时需根据医院的具体情况重点考察柱切换平台的耐久度、经济性、时间复杂度3个重要的评估要素。柱切换技术可实现对体内药物的直接分析,简化前处理过程,使整个过程更加快速准确,临床上可用于药动学和体内药物分析工作。
[Abstract]:Objective: to set up a high performance liquid chromatographic column switching platform in hospital pharmacy department and to simplify the treatment process of complex samples. Methods: using "Column switching" and "column switching" as the key words, we searched the database of "Column switching" and "column switching", searched the related literature about clinical drug analysis column switching from 1970 to 2014, and took the construction of column switching chromatographic network as the main line, and gave different transmission techniques. The problems of one-level column and column switching are summarized and summarized. Results & conclusion: the chromatographic network consists of the first stage column, direct, indirect and reverse transport forms and the selection of detectors. Off-line deproteinization, central cleavage due to better durability, economy and impurity removal ability, suitable for hospital large-throughput sample daily detection. The combination of direct injection, on-line protein removal and recoil transfer technology has become the trend at present. With the maturity of channel restricted packing, this combination will be used more widely. According to the specific conditions of the hospital, it is necessary to investigate three important evaluation factors, namely, durability, economy, and time complexity of the column switching platform. Column switching technique can be used for the direct analysis of drugs in vivo, simplify the pretreatment process, and make the whole process more rapid and accurate. It can be used in pharmacokinetics and in vivo drug analysis in clinic.
【作者单位】：中南大学湘雅医院药学部;中南大学药学院;株洲市恺德医院药学部;
【分类号】：R917;O657.72

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