米索前列醇预防非甾体抗炎药引起的小鼠肠黏膜损伤

发布时间：2018-10-18 20:18

【摘要】：目的探讨米索前列醇对非甾体抗炎药(NSAID)肠黏膜损伤的预防性保护作用。方法双氯芬酸钠灌胃建立NSAID肠黏膜损伤模型。雄性BABL/c小鼠60只随机分组,分别标记为:正常对照组、模型组、(200、400、800μg/kg)米索前列醇组。连续6 d灌胃处理。于第4天下午,除正常组外,其他用5 mg/kg双氯芬酸(10 m L/kg)灌胃处理。第7天对小鼠进行处理:采用FITC标记的葡聚糖检测肠黏膜通透性,分别取小肠组织标本进行HE染色,反转录PCR检测肠黏膜葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)、肿瘤坏死因子α(TNF-α)mRNA的表达,Western blot法检测以上分子的蛋白表达。结果与正常组比较,模型组小肠黏膜损伤严重,黏膜通透性显著升高,小肠黏膜绒毛发生变性、坏死、脱落、炎细胞浸润。而不同浓度米索前列醇给药组的肠黏膜损伤有不同程度的减轻,通透性改善;小肠GRP78蛋白的表达及GRP78、TNF-α、CHOP mRNA的表达与模型组相比明显下调。结论米索前列醇对NSAID肠病的预防有一定的作用。
[Abstract]:Objective to investigate the preventive effect of misoprostol on intestinal mucosal injury induced by non-steroidal anti-inflammatory drug (NSAID). Methods NSAID intestinal mucosal injury model was established by oral administration of diclofenac sodium. 60 male BABL/c mice were randomly divided into normal control group, model group and (200400800 渭 g/kg) misoprostol group. The rats were treated by gavage for 6 days. In the afternoon of the 4th day, except the normal group, the others were treated with 5 mg/kg diclofenac (10 m L/kg). On the 7th day, FITC labeled dextran was used to detect the permeability of intestinal mucosa, and small intestine tissue samples were stained with HE. Reverse transcription-polymerase chain reaction (PCR) was used to detect the expression of glucose-regulated protein 78 (GRP78) in intestinal mucosa, and the expression of (CHOP), tumor necrosis factor 伪 (TNF- 伪) mRNA in C/EBP homologous protein was detected by, Western blot method. Results compared with the normal group, the model group had severe intestinal mucosal injury, increased mucosal permeability, degeneration, necrosis, exfoliation and inflammatory cell infiltration of intestinal villi. However, the intestinal mucosal injury was alleviated and the permeability was improved in different concentrations of misoprostol, and the expression of GRP78 protein and GRP78,TNF- 伪, CHOP mRNA in small intestine were significantly down-regulated compared with the model group. Conclusion Misoprostol has a certain effect on the prevention of NSAID intestinal disease.
【作者单位】：三峡大学第三临床医学院;葛洲坝中心医院消化内科;三峡大学分子生物学研究所;
【基金】：湖北省宜昌市科学研究与开发项目(A11301-45)
【分类号】：R965

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