腹腔注射肿瘤坏死因子α对鼠小肠Cajal间质细胞损伤的研究

发布时间：2018-10-18 20:59

【摘要】：目的探讨腹腔注射肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)对鼠小肠Cajal间质细胞(interstitial cells of Cajal,ICC)的损伤作用。方法 BALB/c裸鼠20只,腹腔注射TNF-α(50μg/kg)后,随机分为4组。分别于注射后即刻(0 h)、6 h、12 h和24 h处死动物,取上段小肠标本。利用HE染色、免疫荧光及透射电镜观察小肠组织、ICC细胞及细胞网络的损伤情况,并通过Western blot方法检测干细胞因子(stem cell factor,SCF)蛋白的表达情况。结果腹腔注射TNF-α后引起小肠组织明显的炎症细胞浸润。12 h后观察到ICC细胞网络结构的缺失,ICC细胞内线粒体肿胀,变形,结构模糊,线粒体嵴结构减少或消失。SCF蛋白表达呈现升高后再降低的变化过程。结论腹腔注射TNF-α对鼠小肠ICC细胞及细胞网络具有损伤作用。
[Abstract]:Objective to investigate the effect of intraperitoneal injection of tumor necrosis factor 伪 (tumor necrosis factor- 伪 (TNF- 伪) on (interstitial cells of Cajal,ICC in rat small intestinal Cajal interstitial cells. Methods Twenty BALB/c nude mice were randomly divided into 4 groups after intraperitoneal injection of TNF- 伪 (50 渭 g/kg). The animals were killed immediately after injection (0 h), 6 h, 12 h and 24 h), and the upper small intestine specimens were taken. HE staining, immunofluorescence and transmission electron microscopy were used to observe the damage of small intestinal tissue, ICC cells and cell network, and the expression of (stem cell factor,SCF protein was detected by Western blot. Results after intraperitoneal injection of TNF- 伪, obvious inflammatory cell infiltration was observed in small intestine. After 12 hours, the loss of ICC cell network, the swelling, deformation and blurred structure of mitochondria in ICC cells were observed. The structure of mitochondrial cristae decreased or disappeared. The expression of SCF protein increased and then decreased. Conclusion Intraperitoneal injection of TNF- 伪 can damage ICC cells and cell network of small intestine.
【作者单位】：哈尔滨医科大学附属第四医院肿瘤外科;哈尔滨市第一医院重症医学科;
【基金】：国家自然科学基金资助项目(81441088) 黑龙江省青年科学基金项目(QC2012C019)
【分类号】：R965

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