福辛普利对胰岛素抵抗小鼠心室重构及脂联素表达的影响

发布时间：2018-11-17 19:13

【摘要】：目的:观察福辛普利干预对胰岛素抵抗(IR)小鼠心室重构及血清和心肌组织中脂联素(APN)表达的影响。方法:随机将40只C57BL/6野生型小鼠分为正常对照组、IR组、Fos7.5组和Fos15组,每组10只。正常对照组给予普通饲料喂养,其余3组均给予高脂饲料喂养,Fos7.5组和Fos15组同时每日分别给予福辛普利7.5mg-1·kg-1·d-1和15mg-1·kg-1·d-1灌胃,共喂养12周。测定体质量、空腹血糖(FPG)及空腹胰岛素(Fins),计算胰岛素抵抗指数(HOMA-IR);采用超声心动图检测左心室后壁厚度、室间隔厚度、左心室射血分数(LVEF)和左室短轴缩短率(LVFS),测定全心质量、左心室质量、左心室质量指数(LVWI);HE染色观察心肌结构微观改变;ELISA法测定血清转化生长因子-β1(TGF-β1)及APN水平,免疫组织化学和Western blot法测定心肌组织中TGF-β1及APN水平。结果:与正常对照组比较,IR组小鼠FPG、Fins及HOMA-IR均升高,左心室后壁厚度及室间隔厚度增加,LVEF和LVFS降低,体质量、全心质量、左心室质量增加,LVWI减小,心肌纤维断裂,排列紊乱,血清及心肌组织中TGF-β1表达升高,APN表达降低(均P0.05)。与IR组比较,Fos7.5组和Fos15组FPG、Fins水平及HOMA-IR均降低,左心室后壁厚度及室间隔厚度均减小,LVEF和LVFS均升高,体质量、全心质量、左心室质量及LVWI均减少,心肌微观结构变化改善,血清及心肌组织中TGF-β1表达降低,APN表达升高,且Fos15组效果更显著(均P0.05)。结论:IR小鼠存在明显心室重构。福辛普利可改善IR小鼠心室重构,且呈剂量依赖性,这种作用可能与APN表达升高有关。
[Abstract]:Aim: to observe the effects of fosinopril on ventricular remodeling and adiponectin (APN) expression in serum and myocardium of insulin-resistant (IR) mice. Methods: forty C57BL/6 wild-type mice were randomly divided into normal control group, IR group, Fos7.5 group and Fos15 group with 10 mice in each group. The normal control group was fed with normal diet, the other three groups were fed with high fat diet. Fos7.5 group and Fos15 group were given 7.5mg-1 kg-1 d-1 and 15mg-1 kg-1 d-1 intragastrically at the same time. Feeding for 12 weeks. Body mass, fasting glucose (FPG) and fasting insulin (Fins), were measured to calculate insulin resistance index (HOMA-IR). Left ventricular posterior wall thickness, interventricular septal thickness, left ventricular ejection fraction (LVEF) and left ventricular shortening rate (LVFS),) were measured by echocardiography. Left ventricular mass index (LVWI);) and left ventricular mass index (LVWI);) were measured by echocardiography. The changes of myocardial structure were observed by HE staining, the levels of serum transforming growth factor- 尾 1 (TGF- 尾 1) and APN were measured by ELISA method, and the levels of TGF- 尾 1 and APN were measured by immunohistochemistry and Western blot method. Results: compared with normal control group, FPG,Fins and HOMA-IR in IR group increased, left ventricular posterior wall thickness and interventricular septal thickness increased, LVEF and LVFS decreased, body mass, whole heart mass, left ventricular mass increased, LVWI decreased and myocardial fiber broke. The expression of TGF- 尾 1 increased and APN decreased in serum and myocardium (P0.05). Compared with IR group, FPG,Fins level and HOMA-IR in Fos7.5 group and Fos15 group decreased, left ventricular posterior wall thickness and ventricular septal thickness decreased, LVEF and LVFS increased, body mass, whole heart mass, left ventricular mass and LVWI decreased. The changes of myocardial microstructure were improved, the expression of TGF- 尾 1 in serum and myocardial tissue decreased, the expression of APN increased, and the effect of Fos15 group was more significant (P0.05). Conclusion: there is obvious ventricular remodeling in IR mice. Fosinopril could improve ventricular remodeling in a dose-dependent manner in IR mice. This effect may be related to the increase of APN expression.
【作者单位】：宁夏医科大学;宁夏医科大学总医院心脏中心干部病房;宁夏医科大学总医院心脏中心功能检查部超声心动图室;
【基金】：国家自然科学基金资助项目(No:81360026)
【分类号】：R965

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