改装滚轮微针促进人增生性瘢痕皮肤对醋酸曲安奈德的吸收

发布时间：2018-11-18 20:23

【摘要】：目的考察改装滚轮微针在人增生性瘢痕皮肤中对醋酸曲安奈德的促透作用。方法采用亚甲蓝和H-E染色法观察不同针长的微针刺入人增生性瘢痕皮肤的形态;建立高效液相色谱法测定醋酸曲安奈德含量;采用洗脱法、匀浆法分别考察给药1、12h时增生性瘢痕皮肤醋酸曲安奈德在体吸收特性;采用经皮水分流失(TEWL)测量法、激光多普勒血流量法考察其皮肤刺激性。结果所优选的针长500μm滚轮微针能有效穿透角质层,形成的微孔分布相对均匀;微针处理1、12h后,分别有27.42%、60.64%药物吸收进入皮肤。给药12h,皮内注射醋酸曲安奈德皮肤中滞留药量较给药1h下降至45.98%,而微针、直接涂布醋酸曲安奈德乳膏分别增加到2.73、4.18倍,微针组皮肤中滞留药量是直接涂布乳膏组的3.56倍(P0.05)。皮内注射12h皮肤药量分布并不均匀,均值为(4.83±5.51)μg,微针组均值为(0.93±0.14)μg,分布相对均匀。TEWL法显示微针处理与皮内注射的皮肤刺激性差异无统计学意义,但激光多普勒血流量法显示皮内注射的皮肤刺激性是微针处理的8.40倍。结论改装滚轮微针处理后涂布醋酸曲安奈德乳膏较皮内注射醋酸曲安奈德在人增生性瘢痕中吸收均匀、皮肤刺激性较小,但其瘢痕治疗效果尚有待进一步验证。
[Abstract]:Objective to investigate the effect of modified roller microneedle on the penetration of triamcinolone acetonide acetate in human hypertrophic scar skin. Methods the morphology of human hypertrophic scar skin was observed by methylene blue and H-E staining, and the content of triamcinolone acetonide acetate was determined by HPLC. The in vivo absorption of triamcinolone acetonide acetate in hypertrophic scar skin was investigated by elution method and homogenate method. The skin irritation of hypertrophic scar skin was investigated by percutaneous water loss (TEWL) method and laser Doppler blood flow method. Results the selected microneedles with a length of 500 渭 m could effectively penetrate the cuticle, and the distribution of the micropores was relatively uniform, and 27.42% of the drugs were absorbed into the skin after treatment for 1h and 12h respectively. After 12 hours of administration, the amount of residual drug in the skin of triamcinolone acetonide acetate decreased to 45.98 after intradermal injection of triamcinolone acetonide acetate, while the microneedle and direct application of triamcinolone acetonide acetate cream increased to 2.734.18 times, respectively. The amount of drug remaining in the skin of the microneedle group was 3.56 times of that of the direct coating cream group (P0.05). The distribution of drug dose in the skin was not uniform at 12 hours after intradermal injection, the mean value was (4.83 卤5.51) 渭 g, and that in the microneedle group was (0.93 卤0.14) 渭 g. There was no significant difference in skin irritation between microneedle treatment and intradermal injection by TEWL method, but the skin irritation by laser Doppler blood flow method was 8.40 times as much as that of micro-needle injection. Conclusion compared with intradermal injection of triamcinolone acetonide acetate, triamcinolone acetonide acetate is absorbed uniformly in human hypertrophic scar, and the skin irritation is less, but the effect of scar treatment needs to be further verified.
【作者单位】：宁夏医科大学研究生院;上海市中医医院药剂科;上海中医药大学岳阳医院药剂科;
【分类号】：R96

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