雌马酚对PC12细胞缺氧/复氧损伤的保护作用及其机制（英文）

发布时间：2021-08-03 21:28

　　目的探讨雌马酚对PC12细胞缺氧/复氧损伤的保护作用及机制。方法以PC12细胞为研究对象,构建体外神经元缺氧/复氧损伤模型,用雌马酚进行干预。MTT法检测细胞活力,比色法测定细胞培养上清液中乳酸脱氢酶（LDH）活性和丙二醛（MDA）含量,免疫细胞化学法检测活性氧（ROS）含量,Western blotting检测缺氧/复氧损伤的PC12细胞内NADPH氧化酶2（gp91phox）和磷酸化Src（p-Src）激酶的表达。结果雌马酚可有效减轻缺氧/复氧引起的PC12细胞损伤,降低缺氧/复氧损伤细胞培养上清液中LDH的活性和MDA的含量,明显抑制ROS的生成及gp91^phox和p-Src的表达。结论雌马酚通过抑制p-Src激酶和gp91^phox表达,减少ROS生成,从而对PC12细胞缺氧/复氧损伤产生保护作用。 

【文章来源】：南方医科大学学报. 2016,36(01)北大核心CSCD

【文章页数】：7 页

【文章目录】：
INTRODUCTION
MATERIAL AND METHODS
    Reagents
    Cell culture and tre atme nt
    Cell viability assay
    LDH activity and MDA conte nt me as ure me nt
    Measure me nt of intrace llular ROS
    Western blotting
    Statistical analys is
RESULTS
    Equol increases viability of PC12 cells exposed to hypoxia/re oxyge nation
    Effects of equol and hypoxia/re oxyge nation on LDH activity and MDA conte nt
    Equol reduce s hypoxia/re oxyge nation-induce d ROS accumulation in PC12 cells
    Effect of equol and hypoxia/re oxyge nation injury on gp91phoxprote in e xpre s s ion
    Effect of equol on Src phos phorylation in PC12 cells subjected to hypoxia/re oxyge nation injury
DISCUSSION



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