桂花类胡萝卜素异构酶基因的克隆及表达分析

发布时间：2018-07-04 10:51

本文选题：桂花 + 花色　；参考：《生物技术通报》2017年06期

【摘要】：利用已构建的桂花‘堰虹桂’花瓣转录组数据库中Unigene序列信息,结合RT-PCR技术对1个桂花类胡萝卜素异构酶基因(Of CRTISO)的最大阅读框进行扩增,并测序验证。生物信息学分析表明Of CRTISO基因含有长为1 842 bp的开放读码框,编码613个氨基酸残基。桂花Of CRTISO与已知胡麻科和茄科植物的CRTISO序列相似性最高,系统进化树分析发现Of CRTISO与芝麻CRTISO(XP_011077785)亲缘关系最近,聚为同一小支。表达分析发现,Of CRTISO基因在桂花‘堰虹桂’花蕾前期花序中的表达量极低,花蕾后期其表达量逐渐增加,初开期达到高峰,而盛开期时,其表达量显著下降。对桂花不同花色品种花瓣中Of CRTISO基因的表达分析表明,桂花初开期Of CRTISO基因的表达量可能影响盛开期各品种花瓣中类胡萝卜素的积累。
[Abstract]:The maximum reading frame of 1 osmanthus carotenoid isomerase isomerase gene (Of CRTISO) was amplified and sequenced using the Unigene sequence information in the database of the Cassia Cassia flower petal transcriptional database of Osmanthus fragrans, which had been constructed and verified by sequencing. The bioinformatics analysis showed that the Of CRTISO gene contained an open reading code frame with a length of 1842 BP, and 613 encoding of the Of CRTISO gene. Amino acid residues. The Of CRTISO of Osmanthus fragrans is the most similar to the CRTISO sequence of the known flax and Solanaceae plants. Phylogenetic tree analysis found that the relationship between Of CRTISO and sesame CRTISO (XP_011077785) was closest to the same small branch. The expression analysis showed that the expression of Of CRTISO gene was very low in the inflorescence of the early bud of the Cassia Cassia flower bud of Osmanthus fragrans. The expression of the Of CRTISO gene in different flower petals of Osmanthus fragrans showed that the expression of Of CRTISO gene may affect the accumulation of carotenoids in the flower petals at the flowering stage.
【作者单位】：浙江农林大学风景园林与建筑学院;
【基金】：国家自然科学基金项目(31501790) 浙江省自然科学基金(LQ15C160004和LQ16C160003) 浙江农林大学科研发展基金人才启动项目(2016FR031)
【分类号】：Q943.2;S685.13

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