同轴静电纺丝法制备具有核-壳纤维结构的多功能敷料

发布时间：2018-02-26 09:21

本文关键词： 同轴静电纺丝多功能敷料胶原体外抗菌细胞黏附　出处：《高等学校化学学报》2016年03期 　论文类型：期刊论文

【摘要】：利用同轴静电纺丝制备了具有核壳结构纳米纤维的未交联敷料,其中纤维内核为载有抗菌药物莫匹罗星的聚己内酯(PCL),外壳则由载有麻醉剂利多卡因的胶原构成;通过京尼平将胶原外壳交联后得到交联敷料.用扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察了未交联敷料的表面形貌和纤维的核壳结构.体外药物释放实验结果表明,在2种敷料中,2种药物在1 h内均出现了突释现象,而在随后的60 h中,2种药物均能从敷料中缓慢释放出来,说明2种敷料均具有较好的持续止痛与抗菌性能.二辛可宁酸(Bicinchonininc acid,BCA)蛋白测试结果表明,未交联敷料外壳上的胶原蛋白能够持续地释放出来.体外细胞培养结果表明,与交联敷料相比,未交联敷料能够更好地促进成纤维细胞L929的黏附和生长,具有更好的促进伤口愈合作用.体外抗菌实验结果显示,负载了莫匹罗星的2种敷料的抗菌性能均明显高于对照组,具有良好的抗菌性能.
[Abstract]:Uncrosslinked dressings with core-shell structure nanofibers were prepared by coaxial electrospinning. The core of the fibers was polycaprolactone containing the antibiotic mupiroxine, and the outer shell was composed of collagen containing lidocaine. The cross-linked dressing was obtained by the cross-linking of collagen shell by genipine. The surface morphology and core-shell structure of the uncrosslinked dressing were observed by scanning electron microscopy (SEM) and transmission electron microscope (TEM). In two kinds of dressings, two kinds of drugs appeared sudden release within 1 h, but in the following 60 h, both drugs could be released slowly from the dressing. The results of Bicinchoncin Inc. BCA protein test showed that the collagen on the uncrosslinked dressing shell could be released continuously. Compared with the cross-linked dressing, the uncrosslinked dressing can promote the adhesion and growth of fibroblasts L929, and promote wound healing. The antibacterial properties of the two kinds of dressing loaded with mupirosin were significantly higher than those of the control group.
【作者单位】：北京理工大学材料学院;结构可控先进功能材料和绿色应用北京市重点实验室;
【基金】：国家大学生创新创业训练计划(批准号:201410007052)资助~~
【分类号】：TQ340.64

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