三相法制备木瓜蛋白酶的过程研究

发布时间：2018-03-10 06:44

本文选题：木瓜蛋白酶　切入点：三相分离　出处：《现代化工》2017年10期 　论文类型：期刊论文

【摘要】：为了高效制备木瓜蛋白酶,利用三相分离法对番木瓜乳粉进行分离纯化。对影响分离纯化的主要因素有机溶剂种类及浓度、硫酸铵质量分数、三相成相时间及pH等进行了研究。实验结果表明,该酶的最佳分离条件为:粗酶液与叔丁醇的体积比为1∶1.5,硫酸铵质量分数为50%,成相时间为60 min,体系pH为7.0。在此条件下使用三相法分离出的木瓜蛋白酶纯化倍数为2.98倍,酶活回收率为76%。
[Abstract]:For the efficient preparation of papain, papaya milk were isolated and purified by phase separation method. The main factors influencing the separation and purification of organic solvent and concentration of ammonium sulfate concentration, three phase time and pH were studied. The experimental results show that the optimum separation conditions of the enzyme: the crude enzyme solution with TERT alcohol volume ratio of 1: 1.5, ammonium sulfate concentration was 50%, the phase time is 60 min, the system pH is isolated by using three-phase 7.0. under the conditions of papain and purification multiples of 2.98 times, the activity recovery was 76%.

【作者单位】：安徽工程大学生物与化学工程学院;微生物发酵安徽省工程研究中心;
【基金】：安徽工程大学微生物制药产业共性研究院开放课题(ZYYJY201503)
【分类号】：Q814

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