化学法制备雄黄量子点及其机理的研究

发布时间：2018-03-15 02:21

  本文选题：雄黄　切入点：量子点　出处：《哈尔滨工业大学》2011年硕士论文　论文类型：学位论文

【摘要】：近年来矿物中药雄黄的抗癌活性在药理基础研究和临床实际应用上都得到了初步的证实,成为除了砒霜外砷类抗癌药的又一新星,与砒霜比其低毒性更是备受关注。但是由于雄黄不溶于水,导致其生物利用度极低,同时使药用机理研究的深入开展困难。研究结果表明减小雄黄的粒径,有助于改善其水溶性,因此纳米雄黄的制备是目前的研究热点。当纳米雄黄的粒径足够小时,由于其组成原子的特性,雄黄纳米粒子具有了荧光量子点的特性-雄黄量子点。雄黄量子点的成功获得不但可以解决雄黄生物利用度极低的难题,还可利用其独特的发光性能解决雄黄体内检测困难的难题,为雄黄药理、毒理的基础理论研究提供了现实可行的条件。 本文采用化学法成功制备出雄黄量子点。通过紫外分析仪和荧光分光光度计等测试手段表征了雄黄量子点光学性能,研究了反应前驱体浓度、反应温度和反应时间对丙烯酸-雄黄量子点发光性能的影响,并提出了工艺条件:反应前驱体浓度为40mg/mL,反应温度为80℃,反应时间为3天。将已经制备的雄黄量子点进行表面修饰,研究结果表明,简单的物理修饰能改善雄黄量子点的发光强度。 实验发现采用化学法制备雄黄量子点,酸的选择是关键。研究了甲酸、草酸和叶酸对雄黄量子点发光性能的影响。利用TEM、UV-vis和PL等分析手段,对不同酸制备的雄黄量子点的发光强度和粒径分布进行了分析,研究结果表明,甲酸和草酸制备的雄黄量子点粒径小、分布窄,发光性能优异。丙烯酸和叶酸制备的雄黄量子点粒径较大、分布较宽,具有浓度效应。 研究了雄黄量子点的可能合成机理:乙二胺与雄黄反应形成分子簇,该分子簇继续生长,成为晶核,使晶体生长顺利进行。酸加入到其中,与乙二胺反应生成酰胺类化合物,改变了体系的环境,同时,改变了乙二胺保护层的结构和性能。
[Abstract]:In recent years, the anticancer activity of realgar, a mineral Chinese medicine, has been preliminarily confirmed in pharmacological basic research and clinical application, and has become a new star of arsenic anticancer drugs besides arsenic. However, because of its insoluble in water, the bioavailability of realgar is very low, and it is difficult to further study the mechanism of medicine. The results show that the size of realgar is reduced. It is helpful to improve its water solubility, so the preparation of nano-realgar is a hot topic. When the particle size of nano-realgar is small enough, because of its atomic properties, Realgar nanoparticles have the properties of fluorescent quantum dots, realgar quantum dots. The success of realgar quantum dots can not only solve the problem of very low bioavailability of realgar. It can also be used to solve the difficult problem of in vivo detection of realgar by using its unique luminescent properties, which provides a practical and feasible condition for the study of the basic theory of realgar pharmacology and toxicology. In this paper, realgar quantum dots were successfully prepared by chemical method. The optical properties of realgar QDs were characterized by UV analyzer and fluorescence spectrophotometer, and the concentration of reaction precursors was studied. The effects of reaction temperature and reaction time on the luminescence properties of acrylic acid realgar quantum dots were studied. The reaction conditions were as follows: the concentration of the precursor was 40 mg / mL, the reaction temperature was 80 鈩，

本文编号：1614005