复凝聚法制备昆虫蜕皮激素微胶囊及其性能研究

发布时间：2018-03-23 00:16

本文选题：复凝聚法　切入点：微胶囊　出处：《东北林业大学》2010年硕士论文　论文类型：学位论文

【摘要】：绿色农药及其制剂开发是目前研究的热点。主要涉及到超高效、低毒(或无毒)、低残留,对环境无污染的化学农药的研究,其中激素类农药是其中的重要方向之一。微胶囊作为一种保护技术,也常常应用于新型农药的制备。本文通过正交实验选取β-蜕皮激素(β-HE)微胶囊的最佳制备工艺条件；利用扫描电子显微镜和光学显微镜对制备的β-HE微胶囊形貌进行表征；研究了β-HE微胶囊的释放行为和光稳定性,并进行了β-HE微胶囊的室内毒力测定实验。主要研究结果如下：通过单因素优化和多因素正交优化实验,确定体系中壁材浓度为3%,芯壁材比为1:1,前期pH值为4.6,后期pH值为8.0为β-HE微胶囊制备的最佳工艺条件。在此条件下,可形成壁膜光滑,大小均匀的球形微胶囊,其平均粒径大小为5μm,载药量与包覆率分别达到68.5%和63.4%。微胶囊的释放行为研究表明,在模拟昆虫中肠内环境条件下以及在模拟田间环境条件下,β-HE微胶囊相对于普通β-HE具有明显的缓释行为。在光降解实验中,β-HE微胶囊相对于普通p-HE具有明显的抗紫外线能力,光稳定性较强。毒力测定实验中,获得了β-HE微胶囊与β-HE原药在喂养试虫3天时的毒力回归方程为：(1)β-HE微胶囊：Y=11.1221+1.7053x；LC50=0.0024(2)β-HE原药：Y=11.4260+1.7546x;LC50=0.0022。选取在取食含有β-HE微胶囊与β-HE原药3天后的杀虫效果,β-HE微胶囊的药效持效时间更长,且杀虫效果较显著。
[Abstract]:The development of green pesticides and their formulations is a hot research topic at present. It mainly involves the research of ultra-high efficiency, low toxicity (or non-toxic, low residue, no environmental pollution) chemical pesticides. As a protective technology, microcapsules are often used in the preparation of new pesticides. In this paper, the optimum preparation conditions of 尾 -ecdysone microcapsules were selected by orthogonal experiment, and the morphology of 尾 -HE microcapsules was characterized by scanning electron microscope (SEM) and optical microscope. The release behavior and photostability of 尾 -HE microcapsules were studied, and the indoor virulence test of 尾 -HE microcapsules was carried out. The main results were as follows:. Through single-factor optimization and multi-factor orthogonal optimization experiments, the optimum conditions for the preparation of 尾 -HE microcapsules were determined as follows: the concentration of wall material was 3%, the ratio of core to material was 1: 1, the pH value was 4.6 in the early stage, and the pH value was 8.0 in the later stage. Under these conditions, the wall membrane was smooth. The average particle size of spherical microcapsules with uniform size is 5 渭 m, and the drug loading and coating efficiency are 68.5% and 63.4%, respectively. The release behavior of microcapsules showed that 尾 -HE microcapsules had a significant slow-release behavior compared with that of ordinary 尾 -HE microcapsules under the conditions of simulated insect midgut environment and simulated field environment. In the experiment of photodegradation, 尾 -HE microcapsules have obvious anti-ultraviolet ability compared with ordinary p-HE, and the photostability of 尾 -HE microcapsules is stronger than that of ordinary p-HE. In the virulence test, The virulence regression equation of 尾 -HE microcapsule and 尾 -HE raw drug was obtained as follows: 1: 1) 尾 -HE microencapsulated microcapsule Y: 11.1221 1.7053xLC500.00242) 尾 -HE original drug: yyun11.4260 1.7546xc500.0022. the insecticidal effect of 尾 -HE microcapsule and 尾 -HE microcapsule was longer than that of 尾 -HE microcapsule after 3 days of feeding with 尾 -HE and 尾 -HE, and the efficacy of 尾 -HE microcapsule was longer than that of 尾 -HE microcapsule. And the insecticidal effect is obvious.
【学位授予单位】：东北林业大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：S482.3

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