醇酶法制大豆油废液中异黄酮的纯化及其抗肿瘤作用研究

发布时间：2018-04-03 06:12

  本文选题：大豆异黄酮　切入点：纯化　出处：《食品工业科技》2016年01期

【摘要】：用有机溶剂萃取和树脂吸附纯化醇酶法制大豆油废液中的异黄酮,用高效液相色谱检测纯化的异黄酮的纯度。以人乳腺癌细胞系MCF-7为模型,检测纯化的大豆异黄酮对MCF-7增殖的影响。结果表明,萃取法粗提的最佳萃取溶剂为乙酸乙酯与正丁醇(1∶1)的混合液,异黄酮提取率为70.47%,纯度为26.45%;树脂吸附纯化的最佳条件为:树脂型号,LSA-8;上样浓度,0.8 mg/m L;上样流速,0.5 BV/h;洗脱液,75%的乙醇;洗脱流速,6 BV/h;洗脱体积,1.5倍上样体积,异黄酮提取率为83.71%,纯度为72.09%。抗肿瘤实验表明,大豆异黄酮浓度达到100 mg/L时,能显著(p0.01)抑制MCF-7细胞的增殖,其作用有一定的剂量依赖性。
[Abstract]:Isoflavones from soybean oil waste liquor were purified by solvent extraction and resin adsorption. The purity of isoflavones was determined by HPLC.The effects of purified soybean isoflavones on the proliferation of human breast cancer cell line MCF-7 were studied.The results showed that the optimum extraction solvent was the mixture of ethyl acetate and n-butanol 1: 1).寮傞粍閰�鎻愬彇鐜囦福�

本文编号：1703955