超临界二氧化碳抗溶剂法制备玉米蛋白基纳米营养物

发布时间：2018-05-16 07:33

  本文选题：超临界二氧化碳 + 超临界抗溶剂法　； 参考：《上海交通大学》2010年硕士论文

【摘要】： 玉米蛋白作为一种天然的生物大分子,具有优秀的生物相容性和生物可降解的特性,而且食物蛋白通常被认为是安全的营养物,其本身也有营养价值。玉米蛋白已广泛用于缓释控释药物的载体材料,其中玉米蛋白微球较为常见。其作为载体材料包埋的药物均具有良好的缓释性能、且表现出抗一定的抗胃蛋白酶分解的特性,是一种不错的肠溶性包衣材料。 近年来,超临界流体抗溶剂技术已成功应用于制备微纳米颗粒,因具备溶剂残留低、产品粒径小、粒度分布可控、反应条件温和、工艺简单等优势,该技术在药物载体领域中的应用受到广泛的关注。 本文主要采用超临界流体提高溶液分散法(SEDS)进行制备玉米蛋白基药物载体,并以叶黄素为模型药物,制备叶黄素-玉米蛋白纳米胶囊。主要研究结果如下: 玉米蛋白空白微球的制备和表征。采用SEDS法从玉米蛋白的丙酮-二甲亚砜混合溶液中制得了玉米蛋白纳米颗粒,考察了温度、压力、溶液浓度、二氧化碳流速和溶液流速对玉米蛋白空白微球形貌和粒径的影响。用扫描电镜、纳米粒度分析、热重分析、差热分析等手段对结果进行表征。实验结果表明,SEDS法可用于制备球形度较好,粒径分布较窄的玉米蛋白微球,所得玉米蛋白微球平均粒径在160-400nm之间。温度、压力、溶液浓度都会影响微粒的粒径,选取不同的工艺组合可制备不同粒径及分布要求的微粒。热重分析和差热分析的结果显示制得的玉米蛋白空白微球较原料稳定性有所提高。 叶黄素-玉米蛋白纳米胶囊的制备和表征。在玉米蛋白空白微球制备的基础上,选用叶黄素作为模型药物,采用SEDS法制备叶黄素-玉米蛋白纳米胶囊。考察了温度、压力、溶液浓度、芯材比和溶液流速对叶黄素纳米胶囊形貌、粒径、载药量和包埋率的影响。用扫描电镜、纳米粒度分析、紫外-可见光谱分析、差热分析、X射线衍射分析等手段对结果进行表征。实验结果表明,SEDS法可制备出较高载药量和包埋率的叶黄素-玉米蛋白纳米胶囊,温度、压力、芯材比、溶液流速都会影响纳米胶囊的粒径、包埋率和载药量。在温度为45℃、压力为10MPa、玉米蛋白溶液浓度为1.5%、芯材比为1:18、溶液流速为1mL/min的最佳条件下制备出的叶黄素纳米胶囊载药量有5.87%,包埋率为60.38%,此时纳米胶囊的平均粒径为205nm。差热分析和X射线衍射分析的结果表明,纳米胶囊中叶黄素以晶体的形式存在,且叶黄素晶体是被包埋在玉米蛋白中形成叶黄素-玉米蛋白纳米胶囊。 最后对全文作了总结,对文章的创新点和不足做了概括并提出后续发展的建议。
[Abstract]:As a kind of natural biological macromolecule, corn protein has excellent biocompatibility and biodegradable properties, and food protein is generally considered as a safe nutrient, and it also has nutritional value. Corn protein has been widely used as a carrier material for sustained release and controlled release drugs, among which corn protein microspheres are more common. The drugs which are encapsulated as carrier materials have good slow-release properties and exhibit anti-pepsin decomposition characteristics. They are a good enteric-soluble coating material. In recent years, supercritical fluid antisolvent technology has been successfully applied to the preparation of microparticles, due to the advantages of low solvent residue, small product size, controllable particle size distribution, mild reaction conditions and simple process. The application of this technology in the field of drug carrier has received extensive attention. In this paper, zeaxanthin based drug carrier was prepared by supercritical fluid enhanced solution dispersion method (SEDSs), and lutein was used as model drug to prepare xanthophyll-corn protein nanocapsule. The main findings are as follows: Preparation and characterization of Corn protein Blank Microspheres. Corn protein nanoparticles were prepared by SEDS from a mixture of acetone and dimethyl sulfoxide of corn protein. The effects of temperature, pressure, concentration of solution, flow rate of carbon dioxide and flow rate of solution on the morphology and particle size of corn protein blank microspheres were investigated. The results were characterized by SEM, nano-particle size analysis, thermogravimetric analysis and differential thermal analysis. The results showed that the method could be used to prepare corn protein microspheres with good spherical size and narrow particle size distribution, and the average diameter of the obtained corn protein microspheres was between 160-400nm. The particle size can be affected by temperature, pressure and solution concentration. The results of thermogravimetric analysis and differential thermal analysis showed that the stability of corn protein blank microspheres was higher than that of raw material. Preparation and characterization of lutein-corn protein nanocapsules. Based on the preparation of corn protein blank microspheres, lutein was selected as model drug and xanthophyll-corn protein nanocapsules were prepared by SEDS method. The effects of temperature, pressure, concentration of solution, ratio of core to material and flow rate of solution on morphology, particle size, drug loading and entrapment rate of lutein nanocapsule were investigated. The results were characterized by scanning electron microscopy (SEM), nano-particle size analysis, UV-Vis spectrum analysis, differential thermal analysis (DTA) and X-ray diffraction (DTA). The results showed that high loading and entrapment rate of xanthophyll-corn protein nanocapsule could be obtained by seds method. Temperature, pressure, ratio of core to material, flow rate of solution could all affect the particle size, entrapment rate and drug loading rate of nanocapsule. When the temperature was 45 鈩，

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