酸降解法制备褐藻寡糖抗氧化性的研究

发布时间：2018-06-15 16:02

本文选题：抗氧化 + 酸解　；参考：《食品工业科技》2016年10期

【摘要】：从褐藻胶中提取出聚甘露糖醛酸和聚古罗糖醛酸,利用盐酸降解聚甘露糖醛酸和聚古罗糖醛酸1、2、6 h分别制备了甘露糖醛酸组分(M_1、M_2和M_3)以及古罗糖醛酸组分(G_1、G_2和G_3),并且以肌肽和甘露糖为对照评估寡糖对DPPH自由基、超氧自由基和羟自由基的清除作用。结果表明,甘露糖醛酸以及古罗糖醛酸对DPPH自由基、超氧自由基具有良好的清除作用,且清除作用随着寡糖中还原糖含量的增加而增加。在DPPH体系中,M_3的清除效果要好于肌肽,G_3的清除效果与肌肽相近。在超氧自由基体系中,M_3的清除作用高于M_2和M_1,而G_3的清除作用略低于肌肽。在羟自由基体系中,甘露糖醛酸和古罗糖醛酸的清除效果低于肌肽和甘露醇。实验表明,酸法制备的褐藻寡糖具有较强的抗氧化能力,且抗氧化效果与寡糖的平均聚合度含量有关。
[Abstract]:Poly mannouronic acid and polyguuronic acid were extracted from alginate. The mannouronic acid fractions, M1M2 and M3, were prepared by hydrochloric acid degradation of polymannouronic acid and polyguuronic acid for 6 h, respectively. The G1G2 and G3S fractions of galuronic acid were prepared, respectively. The DPPH free radicals were evaluated by carnosine and mannose. Scavenging effect of superoxide radical and hydroxyl radical. The results showed that mannouronic acid and uronic acid had a good scavenging effect on DPPH free radical and superoxide free radical, and the scavenging effect increased with the increase of reducing sugar content in oligosaccharides. In DPPH system, the scavenging effect of M _ 3 was better than that of carnosine G3 and the effect was similar to that of carnosine. In the superoxide free radical system, the scavenging effect of M _ S _ 3 is higher than that of M _ S _ 2 and M _ 1, but the scavenging effect of GSP _ 3 is slightly lower than that of carnosine. In hydroxyl radical system, the scavenging effect of mannouronic acid and uronic acid was lower than that of carnosine and mannitol. The results showed that the alginate oligosaccharides prepared by acid method had strong antioxidant capacity and the antioxidant effect was related to the average degree of polymerization of oligosaccharides.
【作者单位】：湖南农业大学生物科学技术学院;中国农业科学院农产品加工研究所农业部农产品加工综合性重点实验室;中国农业大学食品科学与营养工程学院植物源功能食品北京市重点实验室;日本国际农林水产业研究中心;湖南农业大学食品科学技术学院;
【基金】：国家“十二五”科技支撑计划项目(2014BAD04B06) 国家自然科学基金项目(31171738)
【分类号】：TS201.2

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