绿豆粉丝废水生物法制备降血压肽的研究

发布时间：2018-06-22 14:11

  本文选题：绿豆蛋白 + 酶解　； 参考：《湖北工业大学》2011年硕士论文

【摘要】：本文以绿豆粉丝废水为原料,采用酶解技术制备具有降血压功能的ACE抑制肽,利用膜技术对ACE抑制肽进行初步分离纯化;利用大孔吸附树脂对超滤透过液进行静态吸附和解吸,动态吸附和解吸,进一步纯化ACE抑制肽。 1、选取中性蛋白酶、碱性蛋白酶、胃蛋白酶、木瓜蛋白酶对绿豆蛋白进行酶解实验,以水解度和ACE抑制率为指标,结果表明碱性蛋白酶水解度和ACE抑制率均高于其它蛋白酶。经微波处理后的绿豆蛋白的水解度较经热处理后的绿豆蛋白的水解度高,实验确定微波预处理的合适功率为500W,时间为30s。 通过单因素实验和响应曲面法确定碱性蛋白酶最佳水解条件为:底物浓度2%,pH7.5,水解时间120min,加酶量3%,水解温度55℃;碱性蛋白酶最佳水解条件下,ACE抑制率为84.83%,分子量分布比较宽,其水解体系中还存在着较多较大分子量的多肽。 2、以膜通量和ACE抑制率为考察指标,比较了四种不同截留分子量的超滤膜对膜通量和ACE抑制率的影响,结果表明截留分子量3000的超滤膜膜通量相对较大,透过液的ACE抑制率较高达到87.32%。 通过单因素实验和正交实验确定超滤膜的最佳工艺参数:料液温度35℃,超滤时间10min,超滤压力0.08MPa,pH8,在此条件下,平均膜通量达到32.71%。利用高效液相色谱分析超滤透过液分子量分布,发现分子量大部分在700以下,达到了初步分离纯化的目的。 3、以树脂对多肽的吸附性能和吸附率为指标筛选最优树脂,实验表明DA201-C树脂的吸附性能较好,对绿豆多肽的吸附率达到70.45%。采用DA201-C大孔吸附树脂能有效脱去超滤透过液中的盐分,使用浓度为30%,50%,70%,90%的乙醇对超滤透过液进行洗脱,实验结果表明经浓度为70%乙醇洗脱的组分ACE抑制活性最高,ACE抑制率达到91.23%,相比超滤透过液提高了5.78%。对ACE抑制肽达到较好的纯化目的,说明利用大孔吸附树脂处理超滤透过液对降血压肽段起到了一定的富集纯化作用。
[Abstract]:ACE inhibitory peptides with hypotensive function were prepared by enzymatic hydrolysis from mung bean vermicelli wastewater. ACE inhibitory peptides were preliminarily separated and purified by membrane technology, and the macroporous adsorption resin was used for static adsorption and desorption of ultrafiltration permeates. After dynamic adsorption and desorption, ACE inhibitory peptide was further purified. 1. Neutral protease, alkaline protease, pepsin and papain were selected for enzymatic hydrolysis of mung bean protein. The degree of hydrolysis and the inhibition rate of ACE were used as indexes. The results showed that the degree of hydrolysis of alkaline protease and the inhibition rate of ACE were higher than those of other proteases. The hydrolysis degree of mung bean protein after microwave treatment was higher than that of heat treated mung bean protein. The optimum power of microwave pretreatment was 500W and the time was 30s. The optimum hydrolysis conditions of alkaline protease were determined by single factor experiment and response surface method. The optimum hydrolysis conditions were as follows: substrate concentration 2: pH 7.5, hydrolysis time 120 min, enzyme addition 3 min, hydrolysis temperature 55 鈩，

本文编号：2053084