高效液相色谱法制备人面果中二苯甲酮异构体环大叶藤黄醇和异大叶藤黄醇的研究

发布时间：2018-06-24 00:46

  本文选题：π键二苯甲酮异构体 + 环大叶藤黄醇和异大叶藤黄醇　； 参考：《天然产物研究与开发》2017年07期

【摘要】：本研究报道了一种制备人面果中π键二苯甲酮异构体环大叶藤黄醇和异大叶藤黄醇的液相色谱研究方法。实验中通过色谱柱以及流动相考察,在分析色谱柱上实现了液相方法的建立、模拟及筛选试验,并在半制备色谱柱上实现了放大试验和进样量考察。优化后的色谱条件可实现异构体达到2.5的分离度,并在浓度0.2μg/μL时的进样量达到500μL,单体化合物环大叶藤黄醇和异大叶藤黄醇可通过液相色谱、核磁共振DEPT谱及质谱予以确认。该研究表明,常规C_(18)色谱柱能够对π键异构体进行拆分,将加快更多具有生物活性的二苯甲酮单体化合物的发现。
[Abstract]:In this paper, a liquid chromatographic method for preparation of 蟺 -bonded benzophenone isomers from human noodle fruit, cymbiferol and isobalpinol, is reported. In the experiment, the liquid method was established, simulated and screened on the analytical column by means of chromatographic column and mobile phase inspection, and the amplification test and sample quantity investigation were realized on the semi-prepared chromatographic column. The optimized chromatographic conditions can achieve the separation degree of 2.5 isomers, and the injection amount of the isomers reaches 500 渭 L at the concentration of 0.2 渭 g / 渭 L, and the monomers can be confirmed by liquid chromatography, nuclear magnetic resonance spectroscopy and mass spectrometry. The results show that the conventional C _ (18) column can separate 蟺 -bond isomers, which will accelerate the discovery of more bioactive benzophenone monomers.
【作者单位】： 中南民族大学天然产物化学研究室;中南民族大学药学院;中南民族大学化学与材料科学学院;
【基金】：国家自然科学基金(31000157) 湖北省高校优秀中青年科技创新团队计划(T201220)
【分类号】：O657.72;R284.1
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本文编号：2059202