超临界二氧化碳注入法制备活性物复合物的研究

发布时间：2018-07-05 12:22

  本文选题：超临界二氧化碳注入法 + α-生育酚　； 参考：《上海交通大学》2011年硕士论文

【摘要】：生物活性物质常作为功能食品用来提高人体的某些生理机能,但是这些物质通常难溶于水且不稳定、易被氧化,生物利用度很低。将活性物与有特殊物理化学性物的载体制成复合物不仅可以提高活性物的稳定性,还可以增大活性物的生物利用度。传统的复合物制备方法会导致有机溶剂残留并且有可能破坏活性物的生物活性,限制了产品的应用。超临界二氧化碳适中的临界温度和惰性气体性质可以避免热敏性和易氧化物质的破坏;其低粘度,低表面张力和高渗透性以及无需后续处理,大大节省了时间,提高了制备效率。本文选择α-生育酚、叶黄素、亚麻酸乙酯等作为维生素类、类胡萝卜素类、抗氧化剂类以及不饱和脂肪酸类的模型物,以β-环糊精和介孔二氧化硅为载体进行复合物制备研究。本文研究内容和结果如下: 1.以β-环糊精为载体,α-生育酚为模型物,采用超临界二氧化碳注入法制备α-生育酚/β-环糊精固体复合物。考察了注入压力、注入温度和注入时间等参数对α-生育酚负载量的影响以及复合物在水相中的分散性。用紫外分光光度法测定了复合物的负载量,FTIR、DSC和TGA对复合物进行了表征。实验结果表明:随着注入压力和温度的增加,α-生育酚的负载量先增大后减小,当温度为35℃,压力为18MPa,时间为1h时,负载量最高达38.7%;制备的复合物能在水相中均匀分散。 2.以介孔二氧化硅为载体,α-生育酚为模型物,采用超临界二氧化碳注入法制备了α-生育酚/介孔二氧化硅固体复合物。研究过程中,成功制备了不同规格的介孔硅;利用紫外分光光度法分析了注入前后α-生育酚的结构;采用照相机和SEM分析考察了α-生育酚分布和分散情况;考察了过程参数注入压力和温度,不同的二氧化硅对α-生育酚负载量的影响。实验结果表明:随着注入压力和温度的增加,α-生育酚的负载量先增大后减小,当温度为35℃,压力为18MPa,介孔二氧化硅为SBA-15③时,负载量最高达79.88%。 3.以介孔二氧化硅为载体,叶黄素及亚麻酸乙酯为模型物,制备了活性物复合物。并对叶黄素复合物的稳定性进行了研究,研究结果表明制备的复合物中的活性物质稳定性大大提高。 通过本文研究,初步掌握了超临界CO2注入法制备活性物复合物的规律;制备复合物时,过程参数、载体以及活性物对复合物中活性物负载量的影响;建立了制备活性物复合物新工艺。
[Abstract]:Bioactive substances are often used as functional foods to improve some physiological functions of human body, but these substances are usually insoluble in water, unstable, easy to be oxidized and low bioavailability. The stability of the active compound and the bioavailability of the active compound can be increased by making the complex with the carrier with special physical and chemical properties. Traditional complex preparation methods lead to residual organic solvents and may destroy the biological activity of the active compounds, which limits the application of the products. The moderate critical temperature of supercritical carbon dioxide and the properties of inert gases can avoid the destruction of thermosensitivity and oxidizing substances; their low viscosity, low surface tension and high permeability, as well as the need for follow-up treatment, greatly save time, The preparation efficiency is improved. In this paper, 伪 -tocopherol, lutein, ethyl linolenic acid were selected as model materials of vitamins, carotenoids, antioxidants and unsaturated fatty acids. The complexes were prepared with 尾 -cyclodextrin and mesoporous silica as carriers. The research contents and results are as follows: 1. Using 尾 -cyclodextrin as carrier and 伪 -tocopherol as model material, 伪 -tocopherol / 尾 -cyclodextrin solid complex was prepared by supercritical carbon dioxide injection. The effects of injection pressure, injection temperature and injection time on the loading of 伪 -tocopherol and the dispersion of 伪 -tocopherol in aqueous phase were investigated. The complex was characterized by FTIR DSC and TGA. The experimental results show that the loading amount of 伪 -tocopherol increases first and then decreases with the increase of injection pressure and temperature. When the temperature is 35 鈩，

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