交替沉积自组装法制备聚乳酸纳米微粒的多层膜

发布时间：2018-08-12 11:43

【摘要】：交替沉积自组装法由于具有简单快捷、膜结构可控,不受基底形状限制等诸多优点近年来在药物缓释涂层领域受到了广泛的关注。基于交替沉积自组装法人们已经可以将各种不同种类的药物分别载入药物缓释涂层中,但是到目前为止还缺少一种同时加载不同类型的药物到一个多层膜中的有效方法。基于这个问题,本文将载药聚乳酸纳米微粒引入到交替沉积膜中,发展一种制备药物控释涂层的新方法。该方法具有可以将各种不同类型的药物引入到多层膜中,实现单一药物控释涂层对多种不同类型药物的负载等多方面的优点,将为基于交替沉积技术的载药多层膜的制备提供一个新的途径。本文首先利用自乳化溶剂扩散方法制备了包覆有模型药物芘的聚乳酸纳米微粒,并运用扫描电子显微镜、透射电子显微镜、动态光散射等对微球的表面形貌、直径大小、表面电位等进行了表征,然后利用聚乳酸纳米微粒和聚阳离子电解质聚乙酰亚胺的交替沉积制备得到了载药多层膜。用紫外可见光谱对膜的组装过程进行了跟踪,用傅里叶变换红外光谱分析膜的组成,用扫描电子显微镜对膜的形貌进行了观察,对加载到多层薄膜中的芘的含量也用荧光光谱进行了测定。最后进一步研究了交替沉积薄膜中芘的体外释放情况。实验结果表明,该交替沉积膜具有持久地释放芘的预期能力。由于聚乳酸纳米微粒具有良好的生物相容性和生物可降解性,因此该膜在生物材料、组织工程以及基因工程等领域都具有广泛的应用前景。
[Abstract]:The alternating deposition self-assembly method has attracted wide attention in the field of drug sustained-release coatings in recent years because of its advantages such as simple and fast, controllable membrane structure and not limited by substrate shape. Based on the alternative deposition self-assembly method, different kinds of drugs can be loaded into the drug sustained-release coating, but so far there is still a lack of an effective method to load different kinds of drugs into a multilayer film at the same time. Based on this problem, a new method of preparing drug controlled release coatings was developed by introducing drug loaded polylactic acid nanoparticles into alternate deposition films. The method has the advantages of introducing various kinds of drugs into the multilayer film and realizing the loading of a single drug controlled release coating on a variety of different types of drugs. It will provide a new way for the preparation of multilayer film based on alternating deposition technology. In this paper, polylactic acid nanoparticles coated with model drug pyrene were prepared by self-emulsifying solvent diffusion method, and the surface morphology and diameter of the microspheres were investigated by scanning electron microscope, transmission electron microscope and dynamic light scattering. The surface potential was characterized and then the multilayer films were prepared by alternating deposition of polylactic acid nanoparticles and polycationic electrolyte polyimide. The composition of the film was analyzed by Fourier transform infrared spectroscopy (FTIR) and the morphology of the film was observed by scanning electron microscope (SEM). The content of pyrene in multilayer films was also determined by fluorescence spectra. Finally, the in vitro release of pyrene from alternating deposition films was studied. The experimental results show that the film has the expected ability to release pyrene permanently. Due to its good biocompatibility and biodegradability, the membrane has wide application prospects in biomaterials, tissue engineering and genetic engineering.
【学位授予单位】：东北大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：TB383.2

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