沉淀聚合法制备3-吲哚乙酸印迹聚合物微粒及其固相萃取应用

发布时间：2018-11-20 12:01

【摘要】：以3-吲哚乙酸(IAA)为模板分子、丙烯酰胺(AA)为功能单体,采用沉淀聚合法制备了IAA印迹聚合物微粒,研究了致孔剂乙腈的用量及IAA的加入量对印迹聚合物结合性能的影响,采用色谱法对其进行评价。结果表明,当乙腈为37.5 m L,IAA为2 mmol(与AA物质的量比为1∶1)时,制得印迹聚合物P7对IAA的亲和力最大(k=4.24),印迹因子(IF)为14.1,其对IAA展示了最显著的特异识别能力。将P7作为固相萃取柱的填料,研究了分子印迹固相萃取IAA的方法,测得绿豆芽样品的加标回收率为90.6%~92.6%。结果表明,以AA为功能单体沉淀聚合法制备的IAA印迹聚合物颗粒适合作为固相萃取柱填料,可实现实际样品中IAA的选择性分离净化。该文提出的采用适当增大模板分子加入量的方法是制备高亲和力与高选择性印迹聚合物的一条新途径。
[Abstract]:IAA imprinted polymer particles were prepared by precipitation polymerization with 3-indoleacetic acid (IAA) as template molecule and acrylamide (AA) as functional monomer. The effects of the amount of acetonitrile and the amount of IAA on the binding properties of imprinted polymers were studied. The results showed that when acetonitrile was 37.5 mL, IAA was 2 mmol (the ratio to AA was 1:1), the imprinted polymer P7 had the highest affinity to IAA (KN 4.24), and the imprinting factor (IF) was 14.1. It shows the most remarkable recognition ability for IAA. Using P7 as the filler of solid-phase extraction column, the method of molecularly imprinted solid-phase extraction (IAA) was studied. The standard recovery of mung bean sprouts was 90.6% ~ 92.6%. The results showed that the IAA imprinted polymer particles prepared by precipitation polymerization of AA as functional monomers were suitable for solid phase extraction column packing and could realize the selective separation and purification of IAA in practical samples. It is a new way to prepare high affinity and high selectivity imprinted polymers by increasing the amount of template molecule.
【作者单位】：唐山师范学院化学系;河北科技大学生物科学与工程学院;
【基金】：唐山师范学院团队支撑重点项目(2014D07)
【分类号】：O631.3;O658.2

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