酶解法制备菜籽多肽的工艺研究
发布时间：2019-01-07 18:56
【摘要】：本文以菜籽粕为原料,酸处理将菜籽粕脱毒后,采用碱溶酸沉法提纯菜籽蛋白,再用碱性蛋白酶酶解菜籽蛋白制备菜籽多肽,采用单因素逐级优化试验设计,以酶解液中菜籽多肽的含量为指标优化酶解工艺参数。结果表明:酸处理后的硫甙含量下降了84.32%,碱溶酸沉后,蛋白的提取率为76.25%。单因素逐级优化试验的结果表明:碱性蛋白酶酶解的最适条件为底物浓度4%、加酶量7%(E/S)、温度50℃、p H 8.5、酶解时间120 min,该条件下多肽含量为(35.4±0.11)mg/m L。
[Abstract]:Rapeseed protein was purified from rapeseed meal by acid treatment after detoxification, and rapeseed polypeptide was prepared by alkaline protease enzymatic hydrolysis of rapeseed protein. The experimental design was optimized by single factor step by step. The content of rapeseed polypeptide in enzymatic hydrolysis solution was taken as the index to optimize the technological parameters of enzymatic hydrolysis. The results showed that the content of glucosinolate decreased by 84.322.After alkaline acid precipitation, the extraction rate of protein was 76.25%. The results of single-factor stepwise optimization test showed that the optimum conditions for enzymatic hydrolysis of alkaline protease were substrate concentration of 4, enzyme content of 7% (E / S), temperature of 50 鈩，
本文编号：2404013
[Abstract]:Rapeseed protein was purified from rapeseed meal by acid treatment after detoxification, and rapeseed polypeptide was prepared by alkaline protease enzymatic hydrolysis of rapeseed protein. The experimental design was optimized by single factor step by step. The content of rapeseed polypeptide in enzymatic hydrolysis solution was taken as the index to optimize the technological parameters of enzymatic hydrolysis. The results showed that the content of glucosinolate decreased by 84.322.After alkaline acid precipitation, the extraction rate of protein was 76.25%. The results of single-factor stepwise optimization test showed that the optimum conditions for enzymatic hydrolysis of alkaline protease were substrate concentration of 4, enzyme content of 7% (E / S), temperature of 50 鈩，
本文编号：2404013
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