出芽短梗霉产酸性多糖的CTAB法制备工艺优化及其与普鲁兰多糖的性质比较
发布时间：2019-04-04 14:06
【摘要】：采用十六烷基三甲基溴化铵(CTAB)法,制备出芽短梗霉酸性多糖,并比较了其与普鲁兰多糖的溶解性与抗氧化活性。经过单因素实验与正交实验确定制备出芽短梗霉产酸性多糖的最佳工艺条件为:质量浓度3%的CTAB溶液与初始多糖溶液体积比1∶1,初始多糖溶液质量浓度7%,沉淀12 h。该条件下,出芽短梗霉产生的酸性多糖得率为5.01%。该方法保持了出芽短梗霉酸性多糖与普鲁兰多糖的结构完整性,为该多糖的后续研究提供参考。酸性多糖与普鲁兰多糖性质比较实验结果表明,出芽短梗霉产生的中性普鲁兰多糖易溶于水,酸性多糖微溶于水;二者的抗氧化活性在0~1 mg/m L范围内无显著性差异,其还原能力、对超氧阴离子自由基以及羟基自由基清除作用均随溶液浓度的提高而增加,为出芽短梗霉酸性多糖的进一步开发与应用提供理论参考。
[Abstract]:Using cetyltrimethylammonium bromide (cetyltrimethylammonium bromide) (CTAB) method, the acid polysaccharide was prepared and its solubility and antioxidation activity were compared with pullulan polysaccharide. Through single factor experiment and orthogonal experiment, the optimum technological conditions of producing acidic polysaccharide were as follows: the volume ratio of CTAB solution to initial polysaccharide solution was 3%, the mass concentration of initial polysaccharide solution was 7%, the precipitation time was 12 h, the initial polysaccharide concentration was 7%, and the initial polysaccharide solution was precipitated for 12 h. Under these conditions, the yield of acid polysaccharide produced by P. grandiflorum was 5.01%. This method can keep the structure integrity of acid polysaccharide and pullulan polysaccharide of P. grandiflorum, and provide reference for the follow-up study of this polysaccharide. The properties of acid polysaccharide and pullulan were compared. The results showed that the neutral pullulan produced by P. grandiflorum was easily soluble in water, and the acid polysaccharide was slightly soluble in water. There was no significant difference in antioxidant activity between the two groups in the range of 0 ~ 1 mg/m / L, but their reduction ability and scavenging effect on superoxide anion radical and hydroxyl radical increased with the increase of solution concentration. It provides a theoretical reference for the further development and application of acid polysaccharide from P. grandiflorum.
【作者单位】： 江南大学食品学院;
【分类号】：TS201.2
本文编号：2453891
[Abstract]:Using cetyltrimethylammonium bromide (cetyltrimethylammonium bromide) (CTAB) method, the acid polysaccharide was prepared and its solubility and antioxidation activity were compared with pullulan polysaccharide. Through single factor experiment and orthogonal experiment, the optimum technological conditions of producing acidic polysaccharide were as follows: the volume ratio of CTAB solution to initial polysaccharide solution was 3%, the mass concentration of initial polysaccharide solution was 7%, the precipitation time was 12 h, the initial polysaccharide concentration was 7%, and the initial polysaccharide solution was precipitated for 12 h. Under these conditions, the yield of acid polysaccharide produced by P. grandiflorum was 5.01%. This method can keep the structure integrity of acid polysaccharide and pullulan polysaccharide of P. grandiflorum, and provide reference for the follow-up study of this polysaccharide. The properties of acid polysaccharide and pullulan were compared. The results showed that the neutral pullulan produced by P. grandiflorum was easily soluble in water, and the acid polysaccharide was slightly soluble in water. There was no significant difference in antioxidant activity between the two groups in the range of 0 ~ 1 mg/m / L, but their reduction ability and scavenging effect on superoxide anion radical and hydroxyl radical increased with the increase of solution concentration. It provides a theoretical reference for the further development and application of acid polysaccharide from P. grandiflorum.
【作者单位】： 江南大学食品学院;
【分类号】：TS201.2
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