内源乳化法制备溶菌酶肠溶性微胶囊及所用油相回收的研究
发布时间：2019-07-10 15:00
【摘要】：溶菌酶是一种作用于微生物细胞壁的抗菌蛋白,因其较强的抗菌作用被广泛应用于饲料行业,食品行业和医药行业等领域。但在实际应用过程中,溶菌酶作为肠溶性抗菌药物时,因消化道胃酸的影响,使溶菌酶到达肠内时的抗菌性降低。本课题针对此问题,采用微胶囊技术,选择肠溶性壁材—壳聚糖和海藻酸钠,用内源乳化法制备溶菌酶肠溶性微胶囊。同时,除了用石蜡油作为油相,还研究了安全性高的可食用菜籽油作为乳化油相制备微胶囊,使该工艺制备的产品更好的应用于食品、医药等行业。最后,为了使得该制备工艺具有更好的合理性和实际性,本课题首次研究重复利用工艺中乳化的油相,并重复利用,取得了比较理想的效果。主要研究结果如下: 首先采用内源乳化法制备溶菌酶微胶囊。最佳配比:海藻酸钠浓度2.0%,溶菌酶浓度1.5%,壳聚糖浓度0.3%,Span80的添加量为油相体积的1%。正交试验得出制备微胶囊最佳工艺条件:碳酸钙与海藻酸钠质量比7:40,水相与油相体积比30:70,冰醋酸与碳酸钙摩尔比为3:1。最佳制备条件下溶菌酶微胶囊包封率为89.2%,载药量为34.9%;在人工模拟胃液中2h后的释放率为35.5%,在人工模拟肠液中4h后的总释放率为88.9%。在模拟胃液中2h后,包埋后的溶菌酶活性保留率为89.5%,未包埋的仅为31.7%,包埋后的溶菌酶活性保留率比未包埋时明显提高。 然后,用石蜡油和菜籽油分别作为乳化油相制备溶菌酶微胶囊。检测微胶囊性能结果显示分别为:包封率为89.2%和81.7%,载药量为34.9%和26.6%,胃内释放率为35.5%和38.7%,胃肠液中总释放率为88.9%和85.4%,包埋的溶菌酶在胃液中2h后,活性保留率为89.5%和84.7%。用菜籽油制备的微胶囊效果低于用石蜡油制备的。 其次,分别研究了废弃乳状液中石蜡油和菜籽油的回收方法。在石蜡油回收中,比较了加热法,无机盐结合加热法,离心后加热法三种方法对石蜡油的回收效果,结果显示离心后加热法相对较好,在离心转速10000rpm下,离心10min,然后60℃水浴5min,油回收率为98.2%。与石蜡油相比,菜籽油相对更容易进行回收,通过高速离心法,短时间内即可完成对菜籽油的回收,条件为离心转速为10000rpm,离心时间为10min。 最后,研究了石蜡油和菜籽油的重复利用。用回收的石蜡油和菜籽油制备微胶囊,所得到的微胶囊的性能明显下降。
[Abstract]:The lysozyme is an antibacterial protein acting on the cell wall of the microorganism, and is widely used in the fields of feed industry, food industry and medicine industry because of its strong antibacterial effect. In the actual application, however, when the lysozyme is used as the enteric-soluble antibacterial agent, the antibacterial property of the lysozyme to the intestine is reduced due to the influence of the gastric acid in the digestive tract. In this paper, the micro-capsule technology was used to select the enteric-soluble wall material, chitosan and sodium alginate, and the enteric-soluble microcapsule was prepared by the internal emulsification method. At the same time, in addition to using paraffin oil as the oil phase, the edible rapeseed oil with high safety is also studied as the emulsified oil phase to prepare the microcapsule, so that the product prepared by the process is better applied to the industries of food, medicine and the like. Finally, in order to make the preparation process more reasonable and practical, the oil phase emulsified in the process is studied for the first time, and the application is repeated, and the ideal effect is obtained. The main results are as follows: in that first place, the lysozyme is prepare by using the endogenous emulsification method, The optimal ratio of the capsule is as follows: the concentration of the sodium alginate is 2.0%, the concentration of the lysozyme is 1.5%, the concentration of the chitosan is 0.3%, and the amount of the Span 80 is the volume of the oil phase. The optimum conditions for preparing the microcapsules were obtained by orthogonal test: the mass ratio of calcium carbonate to sodium alginate was 7:40, the volume ratio of the aqueous phase to the oil phase was 30:70, and the molar ratio of glacial acetic acid to calcium carbonate was 3. 1. The entrapment efficiency of the lysozyme was 89.2% and 34.9% in the best preparation condition. The release rate was 35.5% after 2 h in the artificial simulated gastric juice, and the total release rate after 4 h in the artificial simulated intestinal fluid was 88. After 2 h of the simulated gastric juice, the active retention of the embedded lysozyme was 89.5%, the non-embedded lysozyme activity was 31.7%, and the retention rate of the embedded lysozyme was higher than that of the non-embedded lysozyme. and then, the paraffin oil and the rapeseed oil are used as the emulsified oil phase to prepare the solution, The results showed that the encapsulation rate was 89.2% and 81.7%, the drug-loading rate was 34.9% and 26.6%, the intragastric release rate was 35.5% and 38.7%, the total release rate in the gastrointestinal fluid was 88.9% and 85.4%, and the retained lysozyme was 89.5% after 2 h of gastric juice. 84.7%. The effect of microencapsulation prepared with rapeseed oil is lower than that of the used stone. The preparation of wax oil was carried out. Then, the paraffin oil in the waste emulsion and the paraffin oil were studied. The recovery of the rapeseed oil was compared in the recovery of paraffin oil. The recovery of the paraffin oil was compared with the heating method, the inorganic salt combination heating method and the post-centrifugal heating method. The results show that the heating method is relatively good after the centrifugation, and the centrifugal rotation speed is 10,000 rpm, the centrifugal speed is 10 min, then the water bath at 60 鈩，
本文编号：2512672
[Abstract]:The lysozyme is an antibacterial protein acting on the cell wall of the microorganism, and is widely used in the fields of feed industry, food industry and medicine industry because of its strong antibacterial effect. In the actual application, however, when the lysozyme is used as the enteric-soluble antibacterial agent, the antibacterial property of the lysozyme to the intestine is reduced due to the influence of the gastric acid in the digestive tract. In this paper, the micro-capsule technology was used to select the enteric-soluble wall material, chitosan and sodium alginate, and the enteric-soluble microcapsule was prepared by the internal emulsification method. At the same time, in addition to using paraffin oil as the oil phase, the edible rapeseed oil with high safety is also studied as the emulsified oil phase to prepare the microcapsule, so that the product prepared by the process is better applied to the industries of food, medicine and the like. Finally, in order to make the preparation process more reasonable and practical, the oil phase emulsified in the process is studied for the first time, and the application is repeated, and the ideal effect is obtained. The main results are as follows: in that first place, the lysozyme is prepare by using the endogenous emulsification method, The optimal ratio of the capsule is as follows: the concentration of the sodium alginate is 2.0%, the concentration of the lysozyme is 1.5%, the concentration of the chitosan is 0.3%, and the amount of the Span 80 is the volume of the oil phase. The optimum conditions for preparing the microcapsules were obtained by orthogonal test: the mass ratio of calcium carbonate to sodium alginate was 7:40, the volume ratio of the aqueous phase to the oil phase was 30:70, and the molar ratio of glacial acetic acid to calcium carbonate was 3. 1. The entrapment efficiency of the lysozyme was 89.2% and 34.9% in the best preparation condition. The release rate was 35.5% after 2 h in the artificial simulated gastric juice, and the total release rate after 4 h in the artificial simulated intestinal fluid was 88. After 2 h of the simulated gastric juice, the active retention of the embedded lysozyme was 89.5%, the non-embedded lysozyme activity was 31.7%, and the retention rate of the embedded lysozyme was higher than that of the non-embedded lysozyme. and then, the paraffin oil and the rapeseed oil are used as the emulsified oil phase to prepare the solution, The results showed that the encapsulation rate was 89.2% and 81.7%, the drug-loading rate was 34.9% and 26.6%, the intragastric release rate was 35.5% and 38.7%, the total release rate in the gastrointestinal fluid was 88.9% and 85.4%, and the retained lysozyme was 89.5% after 2 h of gastric juice. 84.7%. The effect of microencapsulation prepared with rapeseed oil is lower than that of the used stone. The preparation of wax oil was carried out. Then, the paraffin oil in the waste emulsion and the paraffin oil were studied. The recovery of the rapeseed oil was compared in the recovery of paraffin oil. The recovery of the paraffin oil was compared with the heating method, the inorganic salt combination heating method and the post-centrifugal heating method. The results show that the heating method is relatively good after the centrifugation, and the centrifugal rotation speed is 10,000 rpm, the centrifugal speed is 10 min, then the water bath at 60 鈩，
本文编号：2512672
本文链接：https://www.wllwen.com/shekelunwen/minzhuminquanlunwen/2512672.html
