蛋鸡不同产蛋期FGF23-Klotho及其肾脏作用因子表达变化研究

发布时间：2017-12-27 18:23

本文关键词：蛋鸡不同产蛋期FGF23-Klotho及其肾脏作用因子表达变化研究　出处：《沈阳农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：钙磷作为蛋鸡骨骼和蛋壳的重要组成成分,与蛋鸡蛋壳品质密切相关。随着产蛋期的延长,由于蛋鸡钙磷代谢紊乱而导致蛋壳品质下降是制约现代蛋鸡集约化养殖业发展的主要问题之一。成纤维细胞生长因子23(FGF23)属于成纤维细胞生长因子家族,主要由骨细胞和成骨细胞合成分泌,是新近发现的体内调节钙磷代谢的重要因子。现有文献表明,FGF23可能就是哺乳动物体内肠道-肾脏-甲状旁腺-骨骼之间调控机体钙磷代谢的偶联因子。一般认为FGF23主要在肾脏与肾小管上皮细胞膜上的Klotho-FGFRs(成纤维生长因子受体)复合物特异性结合后通过激活MAPK信号通路调节肾小管对钙磷的重新收。但目前,FGF23-Klotho在家禽体内的作用机制尚不清楚。本文通过RT-PCR方法检测了FGF23和KlothomRNA在产蛋鸡胸肌、骨骼肌、肾脏、心脏、肝脏、骨关节、骨、甲状旁腺、肾上腺、蛋壳腺、卵巢、下丘脑、垂体和十二指肠组织中的表达情况;利用实时荧光定量RT-PCR法检测了不同产蛋期骨组织中FGF23 mRNA及肾脏中FGF23-Klotho作用相关因子(Klotho、FGFR1、FGFR3、FGFR4、TRPV5、TRPV6、NCX1、PMCA1b、NaPi-Ⅱa、CaBP-D28k和Cyp24a1)的表达水平;常规方法检测蛋壳品质,探讨不同产蛋期肾脏中FGF23-Klotho作用因子表达变化研究,以期为今后进一步研究FGF23和Klotho在蛋鸡生产中的作用提供前期基础。试验结果证明:1.蛋鸡骨关节、卵巢和肝脏组织中存在FGF23mRNA表达;Klotho在蛋鸡垂体、心脏、十二指肠、卵巢、蛋壳腺、肾脏、肾上腺、甲状旁腺和下丘脑等组织中均有广泛表达。2.产蛋前期和产蛋高峰期蛋鸡骨关节FGF23 mRNA表达水平显著高于产蛋后期;产蛋高峰期肾脏中FGFR1、FGFR3、FGFR4、CaBP-D28k及PMCA1b mRNA表达水平显著高于产蛋前期;产蛋后期TRPV5和Cyp24a1 mRNA表达水平显著低于产蛋前期。3.产蛋后期的蛋壳厚度、蛋壳强度以及蛋壳比例均显著低于产蛋前期和产蛋高峰期。本试验结果表明,产蛋后期蛋壳品质的降低与肾脏对钙重吸收能力下降密切相关。
[Abstract]:As an important component of the skeleton and eggshell of laying hens, calcium and phosphorus are closely related to the quality of egg and egg shell. With the prolongation of laying period, the quality of eggshell resulting from the disorder of calcium and phosphorus in laying hens is one of the main problems that restrict the development of modern laying hens. Fibroblast growth factor 23 (FGF23) is a fibroblast growth factor family, mainly composed of bone cells and osteoblasts. It is a newly discovered important factor regulating calcium and phosphorus metabolism in vivo. The existing literature indicates that FGF23 may be the coupling factor that regulates the metabolism of calcium and phosphorus between the intestinal - kidney - parathyroid - bone in the mammalian body. It is generally believed that FGF23 specifically regulates the recovery of calcium and phosphorus in renal tubules by activating MAPK signaling pathway after binding to the Klotho-FGFRs (fibroblast growth factor receptor) complex on the membrane of kidney and renal tubular epithelial cell membrane. But at present, the mechanism of FGF23-Klotho in poultry is not clear. This paper examined the expression of FGF23 and KlothomRNA in laying hens muscle, skeletal muscle, kidney, heart, liver, bone, bone, adrenal gland, parathyroid, eggshell, ovary, hypothalamus, pituitary and duodenal tissues were detected by RT-PCR method; the role of FGF23 and mRNA related factor FGF23-Klotho in kidney tissue of different laying period by using real time fluorescence quantitative RT-PCR (Klotho, FGFR1, FGFR3, FGFR4, TRPV5, TRPV6, NCX1, PMCA1b, a, CaBP-D28k and NaPi- II Cyp24a1) expression level; conventional method for detection of eggshell quality, to investigate the changes of the function of FGF23-Klotho in the kidney of expression of different laying period, provide a basis for further research on FGF23 and Klotho in laying hens in order to. The results showed that: 1., there was FGF23mRNA expression in bone, joint, ovary and liver tissues of the laying hens. Klotho was widely expressed in the tissues of layers, such as pituitary, heart, duodenum, ovary, eggshell gland, kidney, adrenal gland, parathyroid gland and hypothalamus. 2. egg laying hens and early osteoarthritis FGF23 mRNA expression level was significantly higher than that of egg laying; the expression of FGFR1, FGFR3, FGFR4 egg, CaBP-D28k PMCA1b and mRNA peak in kidney were significantly higher than that of the early laying period; laying TRPV5 and Cyp24a1 mRNA expression level was significantly lower than that of the Early Laying period. 3. the thickness of eggshell, the strength of eggshell and the proportion of eggshell were significantly lower than the pre - laying period and the peak period of egg production. The results showed that the decrease of eggshell quality was closely related to the decrease of renal calcium reabsorption capacity in the later period of laying.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S831

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