蒙古栎嫩枝扦插繁殖技术及其生根机理的研究

发布时间：2017-12-28 05:23

本文关键词：蒙古栎嫩枝扦插繁殖技术及其生根机理的研究　出处：《沈阳农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验以蒙古栎嫩枝为试材,剪取当年生枝条中上部做插穗,采用单一激素ABT1号、NAA、IBA以及混合激素即IBA+NAA=1:1(V:V)4种生长调节剂,分别采取200 mg·L~(-1)、300mg·L~(-1)、500mg·L~(-1)、1000mg·L~(-1)4个浓度梯度,其中低浓度采用浸泡处理2h、4h和6h,高浓度快速浸蘸10s和30 s,以清水作对照,研究不同种类及浓度的外源植物生长调节剂对蒙古栎扦插繁殖的影响。采用高效液相色谱法对其生根过程中内源吲哚乙酸(IAA)、脱落酸(ABA)、玉米素(ZT)、赤霉素(GA_3)4种激素的动态变化进行测定;同时,测定生根过程中插穗内部营养物质(可溶性糖、淀粉、蛋白、总氮、C/N值)的变化,并对生根过程中插穗的基部进行形态学及解剖学观察,探究蒙古栎嫩枝扦插的生根机理。主要研究结果如下:1.外源生长促进剂均可有效的促进蒙古栎嫩枝愈伤组织的发育,其中IBA1000 mg·L~(-1)和IBA+NAA1000 mg·L~(-1)处理10 s的愈伤组织发育较好,愈伤组织重量可分别达到0.5 g和0.52 g,但均不能有效促进生根,生根率仅为3.3%。2.生根过程中,插穗内源激素IAA和ZT可以促进愈伤组织的发育,进而促进不定根的形成,ABA抑制不定根的生成;较高水平的GA_3对扦插生根起阻碍作用。IAA/ABA和ZT/ABA比值与愈伤组织的发育呈正相关,GA_3/ABA比值与愈伤组织的发育呈负相关。3.在生根过程中,插穗内可溶性糖含量的变化趋势为下降-上升-下降;淀粉和可溶性蛋白含量呈现缓慢积累,然后在不定根表达期迅速被消耗,在不定根伸长阶段呈现上升趋势;总氮含量在扦插前期显著升高,之后降低并趋于平稳,在不定根伸长期缓慢积累;C/N比值愈高,愈有利于生根。4.蒙古栎扦插生根过程中,根原基细胞大约出现在扦插后15-20 d;扦插后40 d不定根开始突破表皮,根原基由皮部或愈伤组织伸出,属于混合生根类型;在60 d左右成活的插穗均形成完整的根系。
[Abstract]:In this experiment, Mongolia oak twigs as tested materials, their shoots in the upper part of cuttings, using a single ABT1, NAA, IBA, hormone and mixed hormone IBA+NAA=1:1 (V:V) 4 kinds of growth regulators, take 200 mg - L~ respectively (-1), 300mg L~ (-1), 500mg L~ (-1), 1000mg L~ (-1) 4 concentration gradient, the low concentration of 2H and 4H by soaking and 6h rapid leaching of high concentration of 10s and s in 30, with water as control. The effects of exogenous plant of different types and concentrations of growth regulators on Cutting Propagation of Mongolia oak. Using high performance liquid chromatography on the rooting process of endogenous indole acetic acid (IAA), abscisic acid (ABA), zeatin (ZT), gibberellin (GA_3) dynamic changes of 4 kinds of hormones were determined; at the same time, measured during Rooting of cuttings internal nutrient substances (soluble sugar, starch, protein, total N, C/N) changes and morphological and anatomical observation on the base of the rooting of cuttings, Rooting Mechanism of cuttings of Quercus in Mongolia. The main results are as follows: the promotion of Mongolia oak shoots callus developed 1. exogenous growth promoters can be effective, the IBA1000 mg L~ (-1) and IBA+NAA1000 mg L~ (-1) 10 s callus growth better, callus weight can reach 0.5 g and 0.52 g, but can not effectively promote rooting the rooting rate was only 3.3%. 2., during the rooting process, the endogenous hormones IAA and ZT can promote the development of callus, further promote the formation of adventitious roots, and inhibit the generation of adventitious roots by ABA. A higher level of GA_3 hinders the rooting of cuttings. The ratio of IAA/ABA to ZT/ABA was positively correlated with the development of callus, and the ratio of GA_3/ABA was negatively correlated with the development of callus. 3. in the rooting process, the change trend of soluble sugar content in the ear plug for down - up - down; the contents of starch and soluble protein showed a slow accumulation, and was quickly consumed in adventitious root expression, the rise in adventitious root elongation stage; total nitrogen content increased significantly in the early and decreased after cutting. Stabilized during adventitious root elongation period of slow accumulation; the ratio of C/N is higher, the more conducive to rooting. 4. Mongolia oak cuttings during rooting, root primordium cells occurs at about 15-20 d after cutting; cutting 40 d after adventitious roots began to break through the epidermis, cortex by root primordium or callus rooting out, belong to the mixed type; about 60 d in the survival of cuttings formed complete root.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S792.186

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