饲粮添加菊糖对肉仔鸡生产性能、消化代谢及非特异性免疫功能的影响

发布时间：2018-01-01 13:08

  本文关键词：饲粮添加菊糖对肉仔鸡生产性能、消化代谢及非特异性免疫功能的影响　出处：《沈阳农业大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 生产性能 消化代谢 免疫功能 菊糖 肉仔鸡

【摘要】：本试验旨在探讨饲粮添加菊糖对肉仔鸡生产性能、消化代谢及非特异性免疫功能的影响。试验设计所采用的是单因素完全随机化设计。选取300只1日龄的爱拔益加(AA+)肉仔鸡,随机的将其分为5组,每组有6个重复,每个重复为10只鸡。试验Ⅰ组为对照组:饲喂基础饲粮,试验Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别在基础饲粮中添加0.05%、0.15%、0.25%、0.5%的菊糖。整个饲养试验为期6周,在此期间各组的饲养管理条件相同,自由的采食与饮水。饲养试验结束之后,每个重复选取1只鸡,采用全收粪的试验方法进行代谢试验,测定相关营养物质的代谢率与代谢能。屠宰前采集血清样本,屠宰后,分离免疫器官,取消化道食糜测定消化酶活性,取十二指肠用于制备组织切片,测定组织形态。试验结果表明:(1)饲粮添加菊糖对肉仔鸡的生长性能无显著影响(P0.05),菊糖添加量为0.05%时肉仔鸡4～6周龄和1～6周龄的平均日采食量最高,分别较对照组提高了 3.53%、2.40%,添加量为0.5%时平均日采食量最低。菊糖添加量为0.5%时各阶段料重比均为最低。(2)饲粮添加菊糖极显著的影响干物质代谢率和表观代谢能(P0.01),显著影响能量的表观代谢率(P0.05),对粗蛋白、粗脂肪等养分代谢率无显著影响(P0.05)。当菊糖添加量为0.5%时干物质的代谢率较对照组提高了 22.21%(P0.01),菊糖添加量为0.25%时能量的表观代谢率和表观代谢能分别较对照组提高了 19.63%(P0.05)和15.71%(P0.01);饲粮添加菊糖显著提高了肉仔鸡十二指肠淀粉酶、空肠胰蛋白酶的活性(P0.05),提高了空肠淀粉酶的活性(P0.05),菊糖添加量为0.5%时空肠胰蛋白酶、十二指肠淀粉酶活性显著高于对照组(P0.05);饲粮中添加0.15%和0.25%的菊糖极显著降低了肉仔鸡胃pH值(P0.01);饲粮添加菊糖显著提高了肉仔鸡十二指肠的绒毛高度(P0.05),降低了十二指肠隐窝深度(P0.05)。菊糖添加量为0.15%时绒毛最高(1317.10 μm)。(3)饲粮添加菊糖,提高了肉仔鸡胸腺、脾脏和法氏囊指数,但各添加水平间无显著差异(P0.05)。0.5%菊糖添加水平组肉仔鸡胸腺、脾脏、法氏囊指数分别较对照组提高了 27.57%、16.28%、36.29%(P0.05);饲料添加菊糖极显著提高了肉仔鸡血清白蛋白的浓度(P0.01)。当菊糖的添加量为0.5%时,血清白蛋白含量(14.35 g/L)极显著地高于对照组(P0.01);饲料中添加菊糖极显著提高了肉仔鸡血清溶菌酶活性(P0.01),显著提高了抗菌肽活性(P0.05),对碱性磷酸酶、补体活性无显著影响(P0.05),0.15%菊糖添加水平组血清溶菌酶活性最高(335.89 U/mL),较对照组提高了 78.55%(P0.01),0.25%菊糖添加水平组抗菌肽的活性最高(347.16 U/mL),较对照组提高了 84.30%(P0.05)。综合以上各指标可以得出,饲粮添加菊糖显著提高了消化酶活性和十二指肠绒毛高度,进而提高了肉仔鸡对饲粮主要养分的代谢率和表观代谢能,提高了非特异性免疫功能,且菊糖的适宜添加量为0.25%～0.5%。
[Abstract]:This experiment was conducted to investigate the dietary inulin on broiler performance, affecting digestion and metabolism and non specific immune function. The experimental design is a single factor completely randomized design. A total of 300 1 day old arbor acres (AA+) broilers were randomly divided into 5 groups, each group had 6 replicates repeat for each, 10 chickens. The first group was the control group: fed the basal diet, test II, III, IV, V group were added in the basic diet 0.05%, 0.15%, 0.25%, 0.5% inulin. The feeding experiment lasted for 6 weeks, during which the feeding and management conditions were the same, free. Food and drinking water. After the end of the experiment, 1 chickens from each replicate, the test method of total fecal collection of metabolic test, determination of nutrient metabolism and metabolic rate. Serum samples collected before slaughter, slaughter, immune organ separation, canceled tract test 瀹氭秷鍖栭叾娲绘，

本文编号：1364627