高效复合微生态制剂的研制及其应用

发布时间：2018-01-03 14:43

本文关键词：高效复合微生态制剂的研制及其应用　出处：《河北工业大学》2015年硕士论文　论文类型：学位论文

【摘要】：微生态制剂是一种替代抗生素的新型产品,以其无残留、无耐药性、无毒副作用、效果显著等优点广泛应用在水产养殖、动物养殖、种植业以及疾病治疗等领域。本研究的内容主要在于筛选新的菌株,制备复合微生态制剂以及针对性地应用于水产养殖水处理。具体研究内容包括:1、从沧州和天津地区采集并筛选出8个有效底泥样品,利用选择培养基筛选出了11株特性菌株。进而对筛选得到菌株进行了初步性能检测,得到4株处理人工废水最佳的菌株,对它们分别编号为21-B、31-A、33-A、41-A。2、通过分子实验和生理生化实验对筛选的菌株进行鉴定,鉴定结果是21-B菌为阪崎肠杆菌(Cronobacter sakazakii),31-A菌为枯草芽孢杆菌(Bacillus subtilis),33-A菌为球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus),41-A菌为蜡状芽孢杆菌(Bacillus cereus)。3、单独对阪崎肠杆菌进行脱氮性能检测,该菌株对亚硝酸盐的去除率为97.6%,对氨氮的去除率高达98.3%。4、将得到的另外3株单菌株进行混合培养。优化的混合培养基为葡萄糖10 g?L-1、酪蛋白10 g?L-1、磷酸氢二钾2 g?L-1和牛肝浸粉1 g?L-1;最适培养温度为37℃,最适生长pH为8.0,最适的转速为120 r?min-1。最优的复合比是31-A:33-A:41-A为2:1:1,即7号组合处理废水的效果最佳。5、将7号混合菌制剂进行应用实验。其中在模拟性能检测中,亚硝酸盐的去除率达75%,氨氮的去除率在40%。在采集水实验中验证得出混合菌剂对水体中COD的去除率在30%左右,并且证明处理的最适条件与菌株最适的生长条件一致。
[Abstract]:Probiotics is a new product to replace antibiotics, with no residue, no resistance, no toxic and side effects, the advantages of widely used in aquaculture, animal breeding, planting and treatment of diseases and other fields. The contents of the research mainly lies in the screening of new strains of probiotics and preparation for the application of water treatment in aquaculture. The research contents include: 1, collected from Cangzhou and Tianjin area and screened 8 sediment samples, using selective medium were screened from 11 strains of characteristic strain. And made a primary performance test of screened strains, 4 strains of artificial wastewater processing strains best. They were named 21-B, 31-A, 33-A, 41-A.2, through experiment and physiological and biochemical experiments in molecular identification of strains, the identification results are 21-B strains of Enterobacter sakazakii (Cronobacter sakazakii), 31- The strain A was Bacillus subtilis (Bacillus subtilis), 33-A acid bacteria Bacillus Lai (Lysinibacillus sphaericus), spherical 41-A strains of Bacillus cereus (Bacillus cereus).3, of Enterobacter sakazakii in nitrogen removal performance test, the strain on the nitrite removal rate was 97.6%, the removal of ammonia the rate of up to 98.3%.4, the other 3 were single strains were mixed culture. The optimization of mixed medium was glucose 10 g? L-1, G L-1, 10 casein? Two potassium hydrogen phosphate 2 g? L-1 and bovine liver extract powder 1 g? L-1; the optimum culture temperature was 37, the optimum growth pH is 8, the optimal speed of 120 R? Min-1. optimal composite ratio of 31-A:33-A:41-A to 2:1:1, 7 combined wastewater treatment effect is the best.5, No. 7 mixed bacteria application in simulation experiment. Performance test, the nitrite removal rate was 75%, the removal rate of ammonia nitrogen in 40%. collection The water experiment showed that the removal rate of COD in the water body was about 30%, and the optimum conditions for the treatment were the same as the optimum conditions for the strain.

【学位授予单位】：河北工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S948

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