硅缓解欧美杨无性系I-214镉胁迫的生理及转录调控机理

发布时间：2018-01-05 01:13

  本文关键词：硅缓解欧美杨无性系I-214镉胁迫的生理及转录调控机理　出处：《沈阳农业大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 硅 镉 I-214杨 生理响应 转录组测序

【摘要】：当前镉(Cd)污染严重影响着农业生态环境和可持续发展。植物修复是Cd污染土壤修复的重要潜在技术。硅(Si)是植物的有益元素,能增强植物抗逆性,在植物修复中具有广泛的应用前景。本文以欧美杨无性系1-214为研究对象,采用Hoagland营养液水培方式,研究Si对Cd胁迫下1-214杨的生理和转录测序,以揭示施加Si缓解1-214杨Cd毒害的机理。主要结论如下:(1)Si通过改善1-214杨的根系活力、加强1-214杨根系对水分和养分的吸收。并通过降低活性氧的生成和脂质过氧化程度,增加可溶性蛋白与Cd的结合和茎部对Cd的吸收来缓解Cd对1-214杨幼苗的毒害作用。(2)非酶抗氧化系统可能不是Si缓解1-214杨幼苗Cd胁迫的主要途径。(3)以pval0.05且Fold change ≥ 2为差异基因的筛选标准。Cd处理组与对照组相比,共有146个差异表达基因,其中86个上调基因,60个下调基因。Cd+Si处理组与Cd处理组相比,共有540个差异表达基因,其中214个上调基因,326个下调基因。(4)差异表达基因GO和KEGG富集分析得出:Cd处理组与对照组相比,主要涉及热休克蛋白、小热激蛋白(HSP20)、细胞色素P450、硫氧还蛋白;Cd+Si处理组与Cd处理组相比,主要涉及转录因子WRKY、GRAS、DELLA、蛋白激酶以及钙结合的EF手结构。实时定量荧光PCR验证结果与测序结果中基因变化趋势基本一致。(5)以对照组SNP和InDel事件发生数量为参照,Cd+Si处理组比Cd处理组的SNP事件数量升高2.45%;InDel事件数量升高3.08%。同时新转录本预测中新外显子区域占了 94.99%。Si可能通过提高SNPs和InDels事件的发生率以及诱导新外显子区域的形成来缓解Cd胁迫对I-214杨正常生长的抑制作用。
[Abstract]:At present, cadmium and cadmium (CD) pollution seriously affect the agricultural ecological environment and sustainable development. Phytoremediation is an important potential technology for CD contaminated soil remediation. SiSi) is a beneficial element of plants. It can enhance plant resistance and has a wide application prospect in phytoremediation. In this paper, the clone 1-214 of American and European poplar was used as the research object, and the method of Hoagland nutrient solution hydroculture was adopted in this paper. The physiological and transcriptional sequencing of poplar 1-214 under CD stress was studied. In order to reveal the mechanism of applying Si to alleviate CD toxicity of 1-214 poplar, the main conclusions are as follows: 1: 1 Si can improve the root activity of 1-214 poplar. The absorption of water and nutrients by the roots of 1-214 poplar was strengthened, and the production of active oxygen and the degree of lipid peroxidation were decreased. To alleviate the toxicity of CD to 1-214 poplar seedlings by increasing the binding of soluble protein to CD and the absorption of CD by stem. Non-enzymatic antioxidant system may not be the main way for Si to alleviate CD stress in poplar seedlings 1-214. Pval0.05 and Fold change 鈮，

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