镉胁迫下牦牛肝脏组织转录组学及蛋白组学研究

发布时间：2018-01-05 07:10

本文关键词：镉胁迫下牦牛肝脏组织转录组学及蛋白组学研究　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：牦牛在青藏高原牧民的日常生活中占有很重要的地位,青海省周边矿区中的镉(Cd~(2+))污染能够通过牦牛的富集作用将镉(Cd~(2+))带入机体,镉的危害非常大,对牦牛肉制品食用的安全性有着严重的影响。因此,研究镉(Cd~(2+))在牦牛体内的生物代谢途径对牦牛肉的食用安全性有着重要的意义。1、镉胁迫牦牛肾脏细胞的试验中,用浓度为0、5、10、20、30μmol/L氯化镉胁迫牦牛肾脏细胞,通过荧光定量PCR技术测得,当镉(Cd~(2+))添加5μmol/L时,肾脏细胞中的金属硫蛋白基因(MT1)有明显的表达,重金属镉能够促进MT1 mRNA的表达,随着重金属离子镉胁迫强度的增大MT1基因的mRNA的表达量就会增加。2、以青海省大通种牛场5-6个月龄的牦牛为研究对象,以饲喂3mg/ml氯化镉溶液的实验组牦牛和空白组的牦牛肝脏组织构建cDNA文库,通过lllumina Hiseq2000高通量测序系统测序,通过的基因的差异性分析,共筛选到表达差异的基因16029个,表达差异显著的基因共有384个,其中有129个基因表达水平明显上调,255个基因表达水平下调。3、通过对牦牛肝脏组织的蛋白质组学分析,共鉴定到3173个蛋白质,并与试验中设置的空白组牦牛肝脏组织做差异蛋白分析,筛选得到差异蛋白104个,其中68个为上调蛋白,36个为下调蛋白。4、牦牛肝脏组织的蛋白组与转录组做关联分析,对表达差异显著的384个基因和表达差异显著的104个蛋白做关联分析,共关联到14个表达有显著差异的蛋白质。14个蛋白分别是异柠檬酸脱氢酶、羰基还原酶、组织相容性抗原、ADP-核糖基化蛋白、结合珠蛋白、膜联蛋白A2、谷胱甘肽转移酶A1、黄曲霉毒素B1醛还原酶、二甲基苯胺单氧酶、法尼基焦磷酸合成酶、雌激素硫酸转移酶、羧酸酯酶3、脂肪酸结合蛋白、UDP-葡萄糖醛酸转移酶2C1。
[Abstract]:Yak plays a very important role in Tibetan Plateau herdsmen's daily life, the mining area in Qinghai Province, CD (Cd~ (2+)) pollution could be enriched through the role of yak cadmium (Cd~ (2+)) into the body, the harm of cadmium is very large, has a serious impact on the safety of yak meat food. Therefore, the study of cadmium (Cd~ (2+)) in the biological metabolic pathway in Yak on food safety of yak meat has important significance of.1 test, cadmium stress in Yak kidney cells, renal cells with the concentration of stress yak 0,5,10,20,30 mol/L cadmium chloride, measured by fluorescence quantitative PCR. When the CD (Cd~ (2+)) added 5 mol/L, metallothionein gene in kidney cells (MT1) have obvious expression of CD can promote the expression of MT1 mRNA, with the expression of MT1 gene will increase the amount of heavy metal cadmium stress intensity of mRNA increased.2 in Qinghai Province Datong cattle farm in 5-6 month old yak as the research object, to construct a cDNA Library in the liver tissues of yak yak experimental group and blank group was fed 3mg/ml cadmium chloride solution, through the lllumina Hiseq2000 high-throughput sequencing sequencing system, through the analysis of differences of gene, were screened 16029 differentially expressed genes, gene expression obviously there are a total of 384, of which 129 genes expression was significantly up-regulated, and 255 genes expression of.3, through the proteomics of yak liver tissue analysis, identified 3173 proteins, and differential protein analysis and test of yak liver tissue in blank group set in the experiment, screened proteins 104, including 68 up-regulated proteins, 36 down regulated protein.4, yak liver tissue protein group and transcriptome do correlation analysis, significant differential expression of 384 genes and expressed significant difference Do the correlation analysis of 104 proteins, 14 were related to the expression of protein.14 protein are isocitrate dehydrogenase, carbonyl reductase, histocompatibility antigen, ADP- ribosylation protein, haptoglobin, annexin A2, glutathione S-transferase A1, aflatoxin B1 aldehyde reductase. Two methyl aniline monooxygenase, farnesyl pyrophosphate synthase, estrogen sulfotransferase, carboxylesterase 3, fatty acid binding protein, UDP- glucuronosyltransferase 2C1.

【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823.85

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