干湿交替条件对土壤中厌氧氨氧化细菌和氨氧化古菌丰度的影响

发布时间：2018-01-12 11:32

本文关键词：干湿交替条件对土壤中厌氧氨氧化细菌和氨氧化古菌丰度的影响　出处：《东华大学》2017年硕士论文　论文类型：学位论文

【摘要】：由于人工合成氮素对自然界输入过量氮素,导致了一些列氮失衡问题。研究氮循环功能微生物的生长控制因素是氮循环研究的热点。厌氧氨氧化细菌(anaerobic ammonium oxidation,Anammox)和氨氧化古菌(ammonia-oxidizing archaea,AOA)这两种新型氮循环功能微生物的发现,是近年氮循环研究领域的历史性突破。土壤的干湿交替对氮循环过程有重要的影响,其中微生物的响应也不同。本文着眼于研究厌氧氨氧化细菌和氨氧化古菌在不同环境中的分布。通过培养实验,探究了干湿交替条件对其丰度影响,同时利用高通量测序技术研究了微生物群落特征。使用荧光定量PCR确定丰度,使用Illumina平台进行高通量测序。研究结果表明:厌氧氨氧化细菌和氨氧化古菌在不同环境中的分布不同。干湿交替条件对厌氧氨氧化细菌和氨氧化古菌丰度的响应不同。稻田土壤及沉积物中微生物群落组成差异明显。干湿交替条件变化促进了微生物群落演替。研究结果加深了对于自然生态系统中氮循环功能微生物的认识。对于改善人工湿地等生态修复工程的设计与运行管理具有指导意义。
[Abstract]:Due to excessive nitrogen synthetic nitrogen on the nature of input, leads to some problems on the controlling factors of nitrogen imbalance. The growth of microbial nitrogen cycle is the research focus of the nitrogen cycle. The anaerobic ammonia oxidation bacteria (anaerobic ammonium, oxidation, Anammox) and ammonia oxidizing archaea (ammonia-oxidizing, archaea, AOA) of the two new functions of microbial nitrogen cycle that is in the nitrogen cycle in the research field of historical breakthrough. The soil wetting has an important influence on the nitrogen cycle, the microbial response is also different. This paper focuses on the research of anaerobic ammonia oxidizing bacteria and ammonia oxidizing archaea in different environments. The distribution through the cultivation experiment, to explore the effect of alternate wetting and drying conditions on the abundance, at the same time, the use of high-throughput sequencing technology to study the microbial community characteristics. Using fluorescence quantitative PCR to determine the abundance of Qualcomm Illumina platform The amount of DNA sequencing. The results show that: the distribution of anaerobic ammonia oxidizing bacteria and ammonia oxidizing archaea in different environments. Alternate response conditions of anaerobic ammonia oxidizing bacteria and ammonia oxidizing archaea. The abundance of different microbial communities in paddy soils and sediments in the difference of composition. Alternate conditions change to promote the study of microbial community succession. The deepening understanding of microbial nitrogen cycling in natural ecosystems. It has guiding significance for the design and operation of management improve the wetland ecological restoration projects.

【学位授予单位】：东华大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S154.3

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