大庆地区某养猪场免疫猪群猪瘟与猪蓝耳病病毒抗体及病原的监测

发布时间：2018-02-24 05:39

本文关键词： 猪瘟猪繁殖与呼吸综合征疫苗免疫监测　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：猪瘟是由猪瘟病毒引起的一种以急性、高死亡率和高度接触性为特征的重要传染病,给世界各国造成了重大的经济损失,被我国列为一类传染病。近些年猪瘟流行呈现出持续性感染和免疫失败等诸多新特点,给防控猪瘟带来一系列挑战。猪繁殖与呼吸综合征也是严重威胁养猪业发展的重要传染病,2006年我国暴发的高致病性猪繁殖与呼吸综合征疫情造成了重大经济损失。目前我国主要采取疫苗免疫方式防控上述两种传染病。本研究利用商业化ELISA试剂盒和RT-PCR抗原检测试剂盒,对大庆地区某规模化养猪场免疫猪群进行了猪瘟与猪蓝耳病的免疫监测及分析,为制定科学合理的疫苗免疫程序提供了科学依据。猪瘟的免疫监测结果显示:(1)经二次免疫组猪瘟抗体阳性率为95.0%,结果远高于一次免疫组(66.1%);为了进一步验证二次免疫效果,对418份猪血清样品进行检测,其CSFV抗体阳性率为91.4%,结果远高于《2013年国家动物疫病强制免疫计划》规定的70%的抗体合格率;抗体离散度为21.15%,处于正常范围;试验证明该规模化猪场猪瘟活疫苗免疫情况良好。(2)对送检的530份猪血清样品进行了检测,其CSFV抗原阳性率为0,18份样品为可疑;可疑样品CSFV抗体阻断率分布无明显规律,进一步经RT-PCR检测确定为抗原阴性。试验证明该规模化猪场猪群未出现带毒现象,猪瘟隐性感染风险较低。猪繁殖与呼吸综合征的免疫监测结果显示:经二次免疫组PRRSV抗体阳性率为98.35%,结果高于一次免疫组(83.93%);为了进一步验证二次免疫效果,对418份猪血清样品进行检测,其PRRSV抗体阳性率为98.33%,其结果远高于《2013年国家动物疫病强制免疫计划》规定的70%的抗体合格率;阳性样品抗体离散度达32.84%,部分猪只S/P值异常,抗体水平偏高,猪群蓝耳病毒感染压力过大。对异常样品进行RT-PCR抗原检测,发现存在野毒感染现象。
[Abstract]:Swine fever is caused by classical swine fever virus is a kind of acute infectious diseases, high mortality and highly important contact characteristics, resulting in significant economic losses to the countries of the world, is listed as a kind of infectious disease. In recent years, classical swine fever epidemic showed persistence of infection and immune failure and many other new features, brings a series of the challenge for the prevention and control of swine fever, porcine reproductive and respiratory syndrome is a serious infectious disease of pig industry development in China in 2006, an outbreak of highly pathogenic porcine reproductive and respiratory syndrome epidemic caused significant economic loss. At present our country mainly adopts the way of the two kinds of immune vaccine prevention and control of infectious diseases. In this study, using commercial ELISA kit and RT-PCR antigen detection kit, on a scale of Daqing area farms pigs were immune and immune monitoring analysis of swine fever and PRRS, to develop a scientific and reasonable vaccination To provide scientific basis for disease program. Results showed that the immune monitoring of swine fever (1) after the two immunization group of CSFV antibody positive rate was 95%, the result is much higher than that of a group (66.1%); in order to further verify the two immunity effects were detected in 418 serum samples, the positive rate of CSFV antibody was 91.4% the result is much higher than that of <2013 antibody, national animal epidemic compulsory immunization plan > provisions of the passing rate of 70%; the antibody dispersion was 21.15% in the normal range; test results show that the classical swine fever live vaccine in good condition. (2) of 530 serum samples of samples were detected, the positive rate of CSFV antigen for the 0,18 samples for suspicious suspicious samples; CSFV antibody blocking rate of distribution, further identified as detected by RT-PCR antigen negative. Experimental results show that the scale of pig does not appear toxic phenomenon, swine plague recessive infection risk is low. Porcine reproductive and respiratory syndrome immune monitoring result showed that after the two immunization group PRRSV antibody positive rate was 98.35%, higher than the results of immunization group (83.93%); in order to further verify the two immunity effects were detected in 418 serum samples, the positive rate of PRRSV antibody was 98.33%, the result is much higher than that of <2013 the national animal disease antibody year compulsory immunization plan > provisions of the passing rate of 70%; antibody positive samples dispersion reached 32.84%, some pigs S/P value anomalies, the antibody level is high, the pressure is too large pig blue ear virus infection. RT-PCR antigen detection of abnormal samples, found wild virus infection.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28

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