营养限制与补偿对蒙古羔羊血常规和瘤胃及盲肠生长发育影响

发布时间：2018-02-28 15:49

本文关键词： 营养限制补偿生长蒙古羔羊血液生理指标细胞连接细胞凋亡形态学　出处：《内蒙古农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验研究营养限制与补偿对蒙古羔羊血常规和瘤胃及盲肠生长发育影响,试验选用30只体况正常,平均体重为20.6 kg的4月龄蒙古羔羊。随机分为对照组(CG)、限制组(RG),营养限制期(60 d)结束后,随机在各组挑选5只进行屠宰,每组剩余10只羊,进入补偿期饲喂,各分成两组,每组各五只羊,CH、RH组饲喂精粗比为5:5的粗饲料为青干草的日粮,CM、RM组饲喂精粗比为7:3粗饲料为秸秆的高精料日粮。1.营养限制与补偿对蒙古羔羊血液生理指标的影响。分别在试验的60 d、90 d、120 d通过对羔羊颈静脉采血,应用血常规自动分析仪进行血常规四类十二项指标测试,对数据结果进行分析。结果显示:营养限制结束,R组WBC显著低于C组(P0.01),其他各项指标均有下降趋势;与对照组(CH、CM)相比,补偿30d,限制各组PLT、WBC均有显著性升高;补偿60d,限制各组HGB均呈现上升趋势。整体补偿阶段限制各组RBC均呈下降趋势。2.营养限制与补偿对蒙古羔羊瘤胃、盲肠形态学影响通过HE染色、显微镜等技术,对瘤胃、盲肠黏膜进行观察,研究营养限制及补偿,对瘤胃、盲肠黏膜上皮形态结构的影响。结果显示:营养限制结束,限制组瘤胃乳头长度显著缩短(P0.01),角质层厚度变薄;盲肠黏膜厚度显著降低(P0.01),杯状细胞显著减少(P0.01),嗜伊红细胞、球状白细胞显著增多(P0.05;P0.01)。补偿后,RH、RM组瘤胃乳头高度及角质层厚度有所上升(P0.05),乳头宽度呈现下降趋势;盲肠RM组嗜伊红细胞显著增多(P0.01),RH组球状白细胞显著增多(P0.01),其余各项指标无显著变化。3.营养限制与补偿对蒙古羔羊瘤胃、盲肠细胞增殖增肥与凋亡的影响利用紫外分光光度计及酶标仪,对瘤胃、盲肠的总DNA含量和总蛋白含量进行测定。结果显示:营养限制结束,瘤胃、盲肠细胞数有所增加(P0.05)。补偿后,瘤胃与盲肠呈现不同的变化:限制组与对照组相比,瘤胃细胞数量增多;盲肠细胞则与限制期数量持平。采用TUNEL法并用显微镜观察试验各阶段瘤胃和盲肠上皮细胞的细胞凋亡。结果显示:营养限制结束,瘤胃、盲肠发现细胞凋亡数量显著增多(P0.01);补偿后,瘤胃、盲肠细胞凋亡率均恢复到正常水平(P0.05)。4.营养限制与补偿对蒙古羔羊瘤胃、盲肠组织细胞连接相关蛋白基因的影响通过实时荧光定量分析小窝蛋白、间隙连接蛋白、紧密连接蛋白,在瘤胃、盲肠内mRNA的表达量。结果显示:胃肠道内环境的差异,营养限制阶段,限制组在盲肠组织的表达量高于瘤胃组织的表达量,且高于对照组。补偿阶段,两种补偿方式对瘤胃、盲肠组织各基因的影响无较大差异。
[Abstract]:The effects of nutritional restriction and compensation on blood routine test and growth and development of rumen and cecum of Mongolian lambs were studied. Mongolian lamb, with an average weight of 20.6 kg, was randomly divided into two groups: control group (CGG), restriction group (RGG), nutrition restriction period (60 days). 5 sheep were slaughtered at random in each group. The remaining 10 sheep in each group were fed into the compensatory period and were divided into two groups. Five sheep in each group were fed with high concentrate diet of 7: 3 and straw. 1. The effect of nutrition limitation and compensation on blood physiological index of Mongolian lamb was studied. The blood samples were collected from the jugular vein of the lamb at 60 days, 90 days and 120 days, respectively. 12 items of blood routine were tested by automatic blood routine analyzer, and the data were analyzed. The results showed that the WBC of group R was significantly lower than that of group C (P 0.01), and the other indexes had a downward trend. After 30 days of compensation, there was a significant increase of RBC in each group, and 60 days of compensation, the HGB of all groups showed an increasing trend. The RBC of all groups was decreased in the whole compensation stage. 2. Nutrition restriction and compensation were used in the rumen of Mongolian lamb. The morphological effects of the caecum were observed by HE staining and microscope. The effects of nutritional limitation and compensation on the morphology and structure of the epithelium of rumen and caecum were studied. In the restricted group, the length of rumen papilla was shortened significantly, the thickness of cuticle became thinner, the thickness of caecum mucosa decreased significantly, and the goblet cells significantly decreased P0.01, eosinophilic erythrocyte. The height of rumen papilla and the thickness of cuticular layer in RHNRM group increased after compensation, and the width of nipple decreased. The eosinophilic erythrocytes in the cecum RM group were significantly increased and the globular white blood cells were increased significantly in the RH group. The other indexes had no significant change. 3. Nutritional restriction and compensation on the rumen of Mongolian lamb. Effects of Cecum Cell Proliferation, Fertilizer and apoptosis the contents of total DNA and total protein in rumen and caecum were measured by ultraviolet spectrophotometer and enzyme marker. The number of cecum cells increased (P0.05A). After compensation, the rumen and caecum showed different changes: compared with the control group, the number of rumen cells in the restricted group increased; The number of cecum cells was the same as that of limiting period. The apoptosis of rumen and cecum epithelial cells was observed by TUNEL method and microscope. The results showed that the end of nutrition restriction, the end of rumen, The number of apoptosis in the caecum was significantly increased (P 0.01), and the apoptotic rate of rumen and caecum cells returned to the normal level after compensation. The expression of mRNA in rumen and caecum by real-time fluorescence quantitative analysis of fossa protein, gap junction protein and tight junction protein. In nutritional restriction stage, the expression of cecum in the restriction group was higher than that in the rumen tissue and higher than that in the control group. In the compensatory stage, there was no significant difference in the effect of the two compensation modes on the genes in rumen and caecum tissues.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S826.5

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