尖孢镰刀菌胡麻专化型的转录组学研究

发布时间：2018-03-10 12:32

本文选题：尖孢镰刀菌胡麻专化型　切入点：转录组　出处：《内蒙古大学》2017年硕士论文　论文类型：学位论文

【摘要】：胡麻枯萎病是一种由尖孢镰刀菌胡麻专化型引起的严重危害胡麻产量的土传真菌性维管束病害。本研究组在患病胡麻植株的根围土壤中分离得到了一株对尖孢镰刀菌胡麻专化型具有明显抑制效果的萎缩芽孢杆菌SF1。本论文运用转录组测序技术,分别以尖孢镰刀菌胡麻专化型菌株和与生防菌SF1共培养的尖孢镰刀菌胡麻专化型菌株为研究对象,对尖孢镰刀菌胡麻专化型菌株的转录组学进行了测定并对测序数据进行分析,得到以下结果:1、首先对SF1发酵液及其无细胞滤液对G56的抑菌效果进行了比较,之后绘制了不同发酵时间SF1发酵液对G56的抑菌率变化曲线,从而确定了本次实验中实验组的处理方法。2、以G56作为对照组,与SF1发酵液进行共培养后的G56作为实验组,对G56进行转录组测序,发现:(1)可变剪切事件中属于SE类型的AS事件数量最多;A5SS、A3SS、RI三类AS事件数量相当,明显少于SE类型AS事件;同时,并无MXE类型AS事件发生。(2)G56 组、SF1_24h 组、SF1_48h 组分别有 42.20%、41.25%、41.51%的基因不表达,对于表达的基因FPKM值处于16～60范围内的基因数量最多,之后依次是FPKM值处于3～15、60、1～3范围内的基因。(3)SF1_24hvsG56发现690个上调基因,581个基因下调。SF1_48hvs G56发现584个上调基因,595个下调基因;其中882个在SF1_24hvsG56与SF1_48h vs G56均有差异表达。(4)SF1_24hvsG56的全部1271个差异表达基因被注释到30个GOTerm和55个涉及下调基因的KEGG通路,以及66个涉及上调基因的KEGG通路。(5)SF1_48h vs G56的全部1179个差异表达基因被注释到25个GO Term和52个涉及下调基因的KEGG通路,以及65个涉及上调基因的KEGG通路。
[Abstract]:Flax wilt is an soil-borne fungal vascular bundle disease caused by Fusarium oxysporum flax specific type, which seriously endangers the yield of flax. Atrophy Bacillus sp. SF _ (1), which has obvious inhibitory effect on the specific type of flax, was used in this paper, which was sequenced by transcriptome technique. Using Fusarium oxysporum flax specific strain and Fusarium flax specific strain co-cultured with biocontrol bacteria SF1 as the research objects, the transcriptome of Fusarium oxalium specific strain was determined and the sequencing data were analyzed. The results are as follows: 1. The bacteriostatic effect of SF1 fermentation broth and its acellular filtrate on G56 was compared, and then the change curve of bacteriostasis rate of SF1 fermentation broth on G56 at different fermentation time was plotted. Therefore, the treatment method of the experimental group was determined. G56 was used as control group and G56 co-cultured with SF1 fermentation broth as experimental group. G56 was sequenced. It was found that the maximum number of as events belonging to SE type in the variable shear events was the same as that of SE type as events, which were significantly less than those of SE type as events, and no MXE type as events occurred. At the same time, there were 42.201.25% of the 41.51% genes not expressed in the MXE type as events. The number of genes whose FPKM value was in the range of 16 ~ 60 was the highest, followed by the gene whose FPKM value was in the range of 3 ~ (15) 560 / 1 ~ (-1). 690 upregulated genes were found to be down-regulated, 581 genes were down-regulated, and 584 genes were down-regulated by SF1 48h vs G56, followed by 595 down-regulated genes. Among them, 882 differentially expressed genes in SF1_24hvsG56 and SF1_48h vsG56. All 1271 differentially expressed genes in the GOTerm 24hvsG56 were annotated to 30 GOTerm and 55 KEGG pathways involving down-regulated genes. All 1179 differentially expressed genes related to up-regulated gene, KEGG pathway and up-regulated gene, were annotated to 25 go Term and 52 down-regulated KEGG pathways, and 65 KEGG pathways related to up-regulated genes.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S435.659;Q943.2

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