基于生理指标年际变化红砂抗旱性优良家系的早期选择

发布时间：2018-03-13 05:14

  本文选题：红砂　切入点：家系选择　出处：《甘肃农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：通过采集甘肃、内蒙、宁夏三个红砂天然分布区内长势良好的红砂种子,并栽植于武威市羊下坝镇(38°24′N,103°9′E)进行种源试验。通过连续两年对25个家系红砂苗木的酶活性、渗透调节物质含量、光合及荧光特性的测定,运用主成分分析法、隶属函数法等方法分别选出3a生和4a生红砂苗木抗旱性优良家系,比较所选出3a生红砂抗旱性优良家系在到了第4年时,是否仍然为抗旱性优良家系,及与抗旱性相关的不同指标间是否存在明显的年际变化,并阐明产生变化的原因。筛选出能够稳定遗传的抗旱性优良家系,指导红砂的种源选择,为干旱荒漠化地区确定抗旱性强的红砂品种提供理论依据及优良的繁殖材料。试验结果如下:1)表观量子效率(AQY)、暗呼吸速率(Rd)、光补偿点(LCP)、光饱和点(LSP)、最大净光合速率(Pn_(max))、PSⅡ最大光化学效率(F_v/F_m)、PSⅡ潜在活性(F_v/F0)、光化学猝灭系数(qP)、非光化学猝灭系数(qN)、PSⅡ实际光化学量子效率Y(Ⅱ)、叶绿素(a+b)(Chl a+b)、叶绿素a/b(Chl a/b)、可溶性糖(SS)、可溶性蛋白(SP)、脯氨酸(Pro)15个指标在家系间差异极显著(p0.01)。2)在3a生红砂苗木抗旱性优良家系的选择中,通过计算3个主成分下不同家系的综合得分进行抗旱性排序,按照30%的入选率,排名前8的家系分别为H SW-1ZZG2-1XKK-4LZ-1ZZG2-3XGG-3ZZG1-1HSZ-3号家系。通过隶属函数值法,按照30%的入选率,筛选出8个抗旱性强的家系,抗旱性由强到弱依次为LZ-1HSW-1XKK-4ZZG2-1XGG-3ZZG2-3ZYGZ-1SD-3号家系。通过隶属函数值法与主成分分析均入选的家系有HSW-1、XKK-4、ZZG2-1、LZ-1、XGG-3、ZZG2-3,重合率占75%,均入选的家系作为选择出的抗旱性优良家系。3)在4a生红砂苗木抗旱性优良家系的选择中,通过计算3个主成分下不同家系的综合得分进行抗旱性排序,按照30%的入选率,排名前8的家系分别为X KK-4HSW-1ZZG2-1XGG-3LZ-1ZZG1-1ZZG2-3HSZ-3号家系,通过隶属函数值法,按照30%的入选率,对平均隶属函数值进行排序,筛选出8个抗旱性强的家系,抗旱性由强到弱依次为XKK-4ZZG2-1HSW-1XGG-3LZ-1ZZ G1-1ZZG2-3SD-3号家系。通过隶属函数值法与主成分分析均入选的家系有XKK-4、ZZG2-1、HSW-1、XGG-3、LZ-1、ZZG1-1、ZZG2-3,重合率占87.5%,用这两种方法中的任何一种,都能评价红砂的抗旱性,以均入选的家系作为选择出的红砂抗旱性优良家系。4)主成分分析和隶属函数值法都能将抗旱性强的家系筛选出来,且两种方法评价得出抗旱性强的家系重合率为在75%以上,所选择的3a生红砂苗木抗旱性优良家系XKK-4、ZZG2-1、HSW-1、XGG-3、LZ-1、ZZG2-3在4a生家系的选择中同样表现优良,指标间年际变化差异性不大,这两年任何一年的早期选择都可作为预测红砂今后抗旱性的依据,筛选出的家系可作为抗旱的优良品种。
[Abstract]:The provenance experiments were carried out by collecting red sand seeds from three natural distribution areas of Gansu, Inner Mongolia and Ningxia, and planting them in Yangxiaba Town, Wuwei City. The enzyme activities of 25 families of red sand seedlings were studied for two consecutive years. The content of osmotic regulating substances, photosynthetic and fluorescence characteristics were determined. Three and four year old red sand seedlings were selected to have good drought-resistance families by principal component analysis and membership function method, respectively. In the fourth year, the three year red sand families with good drought resistance were selected to compare whether they were still good families with drought resistance, and whether there were significant interannual changes among the different indexes related to drought resistance. The reasons for the change were also clarified. Good families with stable inheritance of drought resistance were screened to guide the selection of the provenance of red sand. The experimental results are as follows: apparent quantum efficiency (QE), dark respiration rate (RDX), light compensation point (LCPP), light saturation point (LSPN), maximum net photosynthetic rate (net photosynthetic rate). The maximum photochemical efficiency of PS 鈪，

本文编号：1604964