彩色马蹄莲佛焰苞呈色物质及其分布特征研究

发布时间：2018-03-16 19:37

本文选题：彩色马蹄莲　切入点：花青素苷　出处：《昆明理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：彩色马蹄莲(Zantedeschi hybridSpr)天南星科马蹄莲属原产地非洲,由于其奇特的花型、丰富艳丽的色彩在世界花卉市场广受欢迎,是切花,盆栽的佳品,被誉为21世纪花卉之星。现今用于观赏的马蹄莲多为杂交品种。花色是观赏植物最为主要的观赏性状,直接影响这花卉的市场价值。本研究以27个彩色马蹄莲为材料,运用测色法、组织切片法、色素成分及色素含量分析等方法对彩色马蹄莲佛焰苞色的形成进行研究。经分析研究得出以下结论:(1)使用色差仪对收集到的27个彩色马蹄莲品种进行花色评价,使用聚类分析并结合色空间(CIELab Space)综合分析将彩色马蹄莲分成黑色系(Black group)、紫红色系(Amaranth group)、红色系(Redgroup)、黄色系(Yellow group)、粉白色系(Pink group)、橙色系(Orange group)六大色系,为彩色马蹄莲色系分类与定义提供理论依据。(2)本研究通过徒手制作植物组织切片,对彩色马蹄莲佛焰苞组织结构进行显微观察,分析彩色马蹄横切面解剖结构与呈色之间的关系。结果发现彩色马蹄莲佛焰苞呈扁平状砖形,并且色素细胞层数越多,佛焰苞的颜色越深。红色系与橙色系品种,含有花青素苷与黄酮、黄酮醇,花青素苷存在于上下表皮细胞,黄酮、黄酮醇在中部叶肉组织内富集,两种色素复合产生了不同程度的红色、橙色。(3)本研究通过利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和高效液相色谱-电喷雾离子化-质谱联用(HPLC-ESI-MS)技术,并采用标准品半定量的方法,对所收集到彩色马蹄莲佛焰苞中主要的色素成分及含量进行定量与定性分析。分析彩色马蹄莲黑色品种花青素苷生物合成途径,外部环境对花青素苷积累的影响,色彩表型L*a*b*值与色素成分含量关系显著,I*值与总的花青素苷的含量呈负相关,随花青素苷含量的升高,亮度降低。运用紫外分光法对收集到的佛焰苞中的胡萝卜素进行测量(UV-visiblespectra),结果表明,所收集到的彩色马蹄莲品种中均未检测出胡萝卜素成分,因此可以认定彩色马蹄莲黄色系、橙色系品种,使其呈黄色和橙色的主要色素物质为黄酮、黄酮醇,而不是类胡萝卜素,并且黄度(b*)与黄酮、黄酮醇(TF)的含量呈正相关的关系。(4)利用illumnina/Solexa第二代高通量转录组测序技术,对彩色马蹄莲小黑妞、浪漫、范图拉这三个品种进行转录组测序,共获得446340条高质量转录组短数列,平均长度为785bp。在Nr数据库中有注释的共有29816条Unigene,将所得到的相关映射基因映射到KEGG数据库提供的参考目录中,其中55条序列可能设计彩色马蹄莲花色形成:并结合代谢信息与代谢物质变化研究,阐述彩色马蹄莲花青素苷代谢途径。
[Abstract]:Calla Lily (Zantedeschi hybridSpr) of Araceae Zantedeschia origin in Africa, because of its peculiar flowers, rich bright colors are popular in the world, the flower market is cut flowers, potted flowers Jiapin, known as the twenty-first Century star. Now mostly for ornamental Calla cultivars. Ornamental flower color is the most as the main ornamental characters, which directly affects the flower market value. In this study, 27 Zantedeschia as materials, using colorimetry, histological method, pigment composition and pigment content analysis method of Zantedeschia hasuhotoke spathe color formation were studied. After analysis the following conclusions: (1) the evaluation of the collected color using color difference meter 27 Zantedeschia cultivars, using cluster analysis and combined with the color space (CIELab Space) will be a comprehensive analysis of Zantedeschia into black (Black group), purple red The Department (Amaranth group), red (Redgroup), yellow (Yellow group), white powder (Pink group), orange (Orange group) six colors, and provide a theoretical basis for the classification of Zantedeschia color and definition. (2) this study by hand making plant tissue sections of microscopic observation of Zantedeschia hasuhotoke spathe structure, analysis of anatomical structure and relationship between the cross section of a color. The Calla Zantedeschia hasuhotoke spathe was flat brick, and more layers of pigment cells, spathe color deeper. Red and orange varieties contain anthocyanins and flavonoids., flavonols, anthocyanins present in the upper and lower epidermal cells, flavone, flavonol accumulation in the middle mesophyll tissue, two kinds of orange pigment composite had a different degree of red. (3) this study by using high performance liquid chromatography with photodiode array detection (HPLC -DAD) and high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) technology, and using the standard semi quantitative method, collected pigment composition and content of the main color of horseshoe hasuhotoke spathe in quantitative and qualitative analysis. The analysis of Zantedeschia black species anthocyanin biosynthetic pathway. The influence of external environment on anthocyanin accumulation, the relationship between color value and the phenotype of L*a*b* pigment composition content significantly, I* was negatively correlated with the total anthocyanin content, anthocyanin content increased with brightness, reduced. Using ultraviolet spectrophotometry to collect spathaceous in carotenoids were measured (UV-visiblespectra), the results show that the collected Zantedeschia cultivars were not detected in carotenoid composition, it can be found Zantedeschia yellow, orange varieties, the main pigment yellow and orange The material for flavonoids, flavonols, instead of carotenoids, and yellowness (b*) and flavonoids, Huang Tongchun (TF) was positively correlated to the contents of the relationship. (4) using illumnina/Solexa second generation high-throughput transcriptome sequencing technology of Calla Lily little black girl, romantic figure, fan pull the three varieties of transcriptome sequencing, received a total of 446340 high quality transcriptome short sequence, the average length of 785bp. notes in the Nr database with a total of 29816 Unigene related gene mapping, mapping the KEGG database to provide the reference directory, the 55 sequences may design color Calla flower color formation: combined with metabolism research on information and material metabolism, expounds Calla Lily anthocyanin metabolic pathway.

【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S682.264

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