干露胁迫对三疣梭子蟹存活和应激响应状况的影响

发布时间：2018-06-17 22:54

本文选题：三疣梭子蟹 + 运输方法　；参考：《中国海洋大学》2015年硕士论文

【摘要】：1不同运输方法对三疣梭子蟹存活和生理反应的影响研究了不同运输方法(低温水运、低温直接干露和低温间接干露)下运输3h后,三疣梭子蟹的心率、血清皮质醇、葡萄糖和乳酸含量变化,探讨运输胁迫后梭子蟹的存活和生理反应。结果显示： (1)3h运输结束后,低温间接组中幼蟹成活率仅为85%,低于其它2组；相同运输方式下,成蟹的成活率大于幼蟹。(2)3h运输结束后,三种运输方式中梭子蟹血清皮质醇、葡萄糖和乳酸含量均有相似的变化趋势。干运组中这三种物质的含量显著高于对照组和水运组(P0.05)；而水运组与对照组差异不显著(P0.05)。 (3)干露0.5h时,梭子蟹的心率达到最低值17次/min；干露3h时,梭子蟹的心牢上升到22次/min；3-4h干露期间一直维持在22次/min。研究结果表明,3h可能是梭子蟹的临界干露时长；幼蟹运输应采用低温水运法,短距离运输(小于3h运输时长)成蟹时可选择低温干法运输方式。2干露对三疣梭子蟹抗氧化和应激能力的影响在实验室条件下,研究了干露时间(0.5、1.5和3h)胁迫对三疣梭子蟹(Portunus trituberculatus)抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示： (1)干露时间胁迫对超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均无显著影响(P0.05)；但干露时间显著影响肝胰脏总抗氧化能力(T-AOC)水平、热休克蛋白70(Hsp70)水平和肌肉乳酸含量(P0.05)。 (2)0.5h干露胁迫后,SOD活力在入水恢复4h时达到峰值；MDA含量在入水恢复2-10h内显著高于对照组水平(P0.05)：T-AOC水平在恢复阶段基本维持在对照组水平。1.5h和3h干露后,SOD活力在入水恢复阶段一直维持在对照组水平,且各时间点与对照组差异不显著(P0.05)。1.5h干露后MDA含量在入水恢复阶段呈先升高后降低趋势,各时间点与对照组差异不显著(P0.05)；而3h干露后入水恢复10h时显著增加(P0.05)。1.5h干露后入水恢复4h时,T-AOC水平显著高于对照组(P0.05)：3h干露后入水恢复0.5h时显著升高(P0.05),2-10h内基本恢复到对照组水平。干露时间胁迫后,乳酸含量均在入水恢复0.5h时达到最大值；Hsp70水平均在在入水恢复2-4h内达到峰值。研究结果表明,相比于其它所选指标,T-AOC水平能够较敏感地反映干露时间胁迫对机体的生理状态的影响；SOD和MDA相互配合能够较灵敏地反映不同干露时长后机体在恢复阶段的免疫水平。3三疣梭子蟹对不同温度下的干露胁迫的生理响应在实验室条件下,研究了不同温度(16℃和31℃)下的干露胁迫及胁迫后入水恢复时间对三疣梭子蟹肝胰腺中SOD、MDA、T-AOC、Hsp70水平及肌肉中乳酸含量的影响,干露时长为3h。研究结果显示：(1)干露温度胁迫对MDA及乳酸含量均无显著影响(P0.05),但显著影响SOD、T-AOC和Hsp70水平(P0.05)。(2)16-C下干露胁迫3h后,SOD活力在入水恢复4h时达到峰值,但各时间点与对照组均未达到显著差异(P0.05)。入水恢复4h时,MDA含量显著升高(P0.05)；10h时,基本恢复到对照组水平。入水恢复0.5-4h内,Hsp70表达量显著低于对照组(P0.05)；10h时,Hsp70表达量显著高于对照组水平(P0.05)。 (3)31℃下干露胁迫3h后,SOD活力在入水恢复0.5-2h内显著降低(P0.05)；4h时,基本恢复到对照组水平。入水恢复0-4h内,MDA含量基本维持在对照组水平；10h时,MDA含量达到峰值,与对照组差异显著(P0.05)。在入水恢复0.5h时,Hsp70表达量达到峰值,显著高于对照组(P0.05)；2-10h内,基本维持在对照组水平。 (4)不同温度下的干露胁迫后,T-AOC水平在入水恢复各时间点均显著高于对照组(P0.05)：乳酸含量在入水恢复2h时达到峰值,与对照组差异显著(P0.05)。研究结果表明,低温(16℃)下的干露胁迫有利于梭子蟹保持良好的生理状态,故在干法运输时,应该适当地降低温度。
[Abstract]:1 the effects of different transportation methods on the survival and physiological response of Portunus three were studied. The changes of heart rate, serum cortisol, glucose and lactic acid content of the three Portunus warts after transportation of 3H under different transportation methods (low temperature water transport, direct dry dew and indirect dry dew of low temperature) were studied. The survival and physiological responses of the shuttle crab after transportation stress were discussed. (1) (1) after the end of the transport, the survival rate of young crabs in the low temperature indirect group was only 85%, which was lower than the other 2 groups; the survival rate of the crab was greater than that of the young crab. (2) after the end of the transport, the content of serum cortisol, glucose and lactic acid in the three transport modes of the three types of transportation were similar. The content of the three substances in the dry Transport Group The difference between the control group and the control group (P0.05) was significantly higher than that of the control group (P0.05). (3) when the dry dew 0.5h, the heart rate of the shuttle crab reached the lowest value of 17 times /min; when the dry dew 3h, the heart of the shuttle crab rose to 22 times /min, and the 3-4h dry dew kept in the 22 /min. research results during the 3-4h dry dew show that 3H may be the critical dry dew of the shuttle crab. Long time, young crab transportation should adopt low temperature water transport method, short distance transport (less than 3H transportation time), the effect of.2 dry dew on the antioxidant and stress ability of three wart crabs can be selected. Under the laboratory conditions, the stress of dry dew (0.5,1.5 and 3H) stress on the oxygen resistance of three verruca crabs (Portunus trituberculatus) is studied. The results showed that (1) dry exposure time stress had no significant effect on the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) (P0.05), but the drying time significantly affected the total antioxidant capacity (T-AOC) of hepatopancreas (T-AOC). Level, heat shock protein 70 (Hsp70) level and muscle lactic acid content (P0.05). (2) after 0.5h dry exposure stress, SOD activity reached its peak value when water was restored to 4h, and MDA content was significantly higher than that of control group (P0.05):T-AOC level in the recovery phase (P0.05) in the recovery phase (P0.05) at the level of.1.5h and 3H dry dew in the control group, and SOD vigor was entered into water restorer. There was no significant difference between the control group and the control group at the complex stage, and there was no significant difference between the control group and the control group at each time point (P0.05).1.5h dry dew. The MDA content increased first and then decreased, and the difference between the time points and the control group was not significant (P0.05), while the 3H dry dew after the water resumed 10h significantly increased (P0.05).1.5h dry dew and recovered 4h, T-AOC. The level was significantly higher than that of the control group (P0.05):3h dry dew after the recovery of 0.5h (P0.05), and the 2-10h content was basically restored to the control level. After the exposure time, the lactic acid content reached the maximum when the water was restored to 0.5h, and the Hsp70 level was reached to the peak value in the return of water to 2-4h. The results showed that compared with the other selected fingers, the results showed that The T-AOC level can sensitively reflect the effect of dry exposure time stress on the physiological state of the body, and the interaction between SOD and MDA can sensitively reflect the immune level of the body in the recovery phase of the different dry dew time and.3 three of the shuttle crab under different temperatures under the laboratory conditions. The effect of dry dew stress at temperature (16 and 31 C) and water recovery time after stress on SOD, MDA, T-AOC, Hsp70 level and lactic acid content in muscle and pancreas in the hepatopancreas of three verruca crabs. The results of 3h. study showed that (1) dry dew temperature stress had no significant effect on MDA and lactic acid content (P0.05), but significantly affected SOD, T-AOC and Hsp70. Level (P0.05). (2) after the exposure to 3H under 16-C, the activity of SOD reached its peak value when the water was restored to 4h, but all time points were not significantly different from that of the control group (P0.05). When the water was restored to 4h, the content of MDA increased significantly (P0.05); 10h, basically recovered to the level of the control group. The expression of Hsp70 was significantly lower than that of the control group; Hsp70 expression was significantly lower than that of the control group; while the 10h, the Hsp70 expression was significantly lower than that of the control group. At h, the expression of Hsp70 was significantly higher than that of the control group (P0.05). (3) after the exposure to 3H at 31 C, the activity of SOD decreased significantly (P0.05) in the return of water to 0.5-2h. When 4h, the level of the control group was basically restored to the control group. The MDA content was basically maintained at the level of the control group; 10h, the MDA content reached the peak, and was significantly different from the control group. When the water was restored to 0.5h, the expression of Hsp70 reached a peak value, which was significantly higher than that of the control group (P0.05); in 2-10h, the level of the control group was basically maintained. (4) after the dry dew stress at different temperatures, the T-AOC level was significantly higher than the control group (P0.05) at all time points in the water recovery. The content of lactic acid reached the peak when the water was restored to 2h, and the difference was significant between the control group and the control group. (P0.05). The results show that the dry dew stress at low temperature (16 C) is beneficial to the good physiological state of the shuttle crab, so the temperature should be reduced properly during the dry transportation.
【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S917.4

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