肿瘤坏死因子α308和肿瘤坏死因子α238位点基因多态性与藏族骨关节结核发病关系研究

发布时间：2018-01-13 19:18

本文关键词：肿瘤坏死因子α308和肿瘤坏死因子α238位点基因多态性与藏族骨关节结核发病关系研究　出处：《中国全科医学》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨肿瘤坏死因子(TNF)α308、TNF-α238位点基因多态性与藏族骨关节结核发病的关系。方法采用单纯随机抽样法选取2012年10月—2013年10月在青海大学附属医院关节骨病科住院治疗,根据病史、临床表现、X线检查、细菌学检查及病理组织学检查等确诊为骨关节结核的青海地区藏族患者65例为试验组,选取同时段本院青海地区藏族体检健康者50例为对照组。采集受试者清晨空腹外周静脉血3 ml,提取其白细胞基因组DNA;采用等位基因特异引物聚合酶链反应(AS-PCR)分析TNF-α308、TNF-α238位点基因多态性。结果两组TNF-α308位点等位基因、基因型频率比较,差异有统计学意义(P0.05)。试验组TNF-α308 G/A、A/A基因型频率与对照组比较,差异有统计学意义(χ2=5.85,P0.05)。两组TNF-α238位点等位基因频率比较,差异无统计学意义(χ2=2.33,P0.05);TNF-α238位点基因型频率比较,差异有统计学意义(P0.05)。结论青海地区藏族骨关节结核患者中TNF-α308 A存在过度表达,且TNF-α308位点基因多态性与青海地区藏族骨关节结核的发病可能有关系;TNF-α238位点基因多态性与青海地区藏族骨关节结核的发病可能无关系。
[Abstract]:Objective to study the tumor necrosis factor TNF- 伪 308. The relationship between the polymorphism of TNF- 伪 238 locus and the incidence of Tibetan bone and joint tuberculosis. Methods from October 2012 to October 2013, we selected the joints in the affiliated hospital of Qinghai University from October 2012 to October 2013. Orthopaedic hospitalization. According to the history, X-ray examination, bacteriological examination and histopathological examination, 65 Tibetan patients with bone and joint tuberculosis were selected as the experimental group. At the same time, 50 healthy Tibetans in Qinghai area of our hospital were selected as the control group. The fasting peripheral venous blood was collected in the morning to extract the genomic DNA of white blood cells (WBC). Allele-specific primer polymerase chain reaction (AS-PCR) was used to analyze the polymorphism of TNF- 伪 308 locus. The frequency of TNF- 伪 308 G / A / A / A genotype in the trial group was significantly higher than that in the control group (P < 0.05). The allelic frequency of TNF- 伪 238 locus was not significantly different between the two groups (蠂 2 / 2.33 / P 0.05). The genotype frequency of TNF- 伪 238 locus was significantly different (P 0.05). Conclusion there is over-expression of TNF- 伪 308A in Tibetan patients with bone and joint tuberculosis in Qinghai area. The polymorphism of TNF- 伪 308 gene may be associated with the incidence of Tibetan bone and joint tuberculosis in Qinghai. The polymorphism of TNF- 伪 238 gene may not be associated with the incidence of Tibetan bone and joint tuberculosis in Qinghai.
【作者单位】：青海大学附属医院关节骨病科;
【分类号】：R529.2
【正文快照】： Relationship between Tumor Necrosis Factor-α308 and 238 Polymorphisms and Osteoarticular Tuberculosis in TibetansLI De-chun.Department of Joint,Qinghai University Affiliated Hospital,Xining 810001,China我国是全球22个结核病高负担国家之一,患者人数居世界第

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