肝脏泡型包虫病患者外周血单个核细胞IL-9、PU.1及IRF-4mRNA表达水平的研究

发布时间：2018-01-14 12:16

本文关键词：肝脏泡型包虫病患者外周血单个核细胞IL-9、PU.1及IRF-4mRNA表达水平的研究　出处：《中国病原生物学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨辅助性T细胞9(Th9)相关细胞因子IL-9及转录因子PU.1和IRF-4mRNA在肝脏泡型包虫病患者外周血单个核细胞中的表达及其作用。方法 2012年10月—2014年3月新疆医科大学第一附属医院收治的肝脏泡型包虫病患者(AE组)14例,健康志愿者(HC组)14例,应用实时荧光定量PCR法检测两组受试者外周血单个核细胞中IL-9、PU.1和IRF-4 mRNA的相对表达量,数据分析采用非参数秩和检验两独立样本比较法(M-W法),用Spearman线性相关性检验分析细胞因子和转录因子之间的相关关系。结果肝泡型包虫病组和健康对照组受试者外周血单个核细胞IL-9 mRNA相对表达量分别为2.44911(IQR:0.69268-5.70628)和0.00316(IQR:0.0198-0.00815),差异有统计学意义(P=0.000);PU.1 mRNA相对表达量分别为313.105(IQR:94.80556-548.13140)和0.77147(IQR:0.28829-1.59077),差异有统计学意义(P=0.009);IRF-4 mRNA相对表达量分别为0.04126(IQR:0.01957-0.08204)和21.58347(IQR:8.95431-52.31027),差异有统计学意义(P=0.000),单个核细胞中IL-9mRNA水平与PU.1mRNA呈正相关(r=0.8228,P=0.000),与IRF-4mRNA也呈正相关(r=0.9332,P=0.000)。结论 Th9细胞功能性细胞因子IL-9以及转录因子PU.1和IRF-4参与泡型包虫病发生发展过程,其表达水平上调有利于虫体继续寄生于宿主体内,同时可能与形成泡型包虫慢性肉芽肿有关。
[Abstract]:Objective to investigate the expression of Th 9 in helper T cells. Expression and role of IL-9, PU.1 and IRF-4mRNA in peripheral blood mononuclear cells of patients with hepatic hydatid disease. Patients with hepatic alveolar hydatid disease admitted to the first affiliated Hospital of Xinjiang Medical University from October 2012 to March 2014 (. 14 cases in AE group. The relative expressions of IL-9 PU.1 and IRF-4 mRNA in peripheral blood mononuclear cells (PBMC) of 14 healthy volunteers were detected by real-time fluorescence quantitative PCR. The non-parametric rank sum test and two independent sample comparison methods were used to analyze the data. The correlation between cytokines and transcription factors was analyzed by Spearman linear correlation test. Results IL-9 of peripheral blood mononuclear cells in patients with hepatic alveolar hydatidosis and healthy control subjects. The relative expression of mRNA was 2.44911 (. IQR: 0.69268-5.70628 and 0.00316IQR: 0.0198-0.00815). The difference was statistically significant. The relative expression levels of PU.1 mRNA were 313.105 (IQR: 94.80556-548.13140) and 0.77147 (respectively). IQR: 0.28829-1.59077. The difference was statistically significant (P < 0. 009). The relative expression levels of IRF-4 mRNA were 0.04126 and 21.58347, respectively. IQR: 8.95431-52.31027). The level of IL-9mRNA in mononuclear cells was positively correlated with PU.1mRNA. There was also a positive correlation with IRF-4mRNA. Conclusion the functional cytokines IL-9 and transcription factors PU.1 and IRF-4 of Th9 cells are involved in the occurrence and development of hydatid disease. The upregulation of its expression may be associated with the formation of chronic granuloma of hydatid hydatid.
【作者单位】：新疆医科大学第一附属医院消化血管外科中心肝脏·腹腔镜外科;新疆医科大学第一附属医院临床医学研究院新疆包虫病基础医学重点实验室;新疆医科大学第一附属医院超声科;
【基金】：国家自然科学基金项目(No.81160201,U1303222) 国家临床重点专科-地方病(包虫病)建设项目教育部“长江学者与创新团队发展计划”项目(No.IRT1181)
【分类号】：R532.32
【正文快照】： 包虫病(hydatid disease)又称棘球蚴病(echino-断,并经外科手术病理检查确认(诊断均依照WHOcoccosis),是棘球绦虫的幼虫寄生在人体引起的一种包虫病非官方工作组制订的分型、分期诊断标准)。健人畜共患的寄生虫疾病,主要分为由细粒棘球绦虫康对照组(HC组)中男8例,女6例,年龄28

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