乙型肝炎病毒外膜大蛋白意义分析及与慢性乙型肝炎患者病毒复制的相关性研究

发布时间：2018-01-20 04:16

本文关键词： 乙型肝炎病毒外膜大蛋白慢性乙型肝炎乙型肝炎E抗原相关性　出处：《国际检验医学杂志》2016年23期 　论文类型：期刊论文

【摘要】：目的通过分析乙型肝炎病毒(HBV)感染患者血清免疫学标志物乙型肝炎病毒外膜大蛋白(HBV-LP)、HBV-DNA及乙型肝炎病毒标志物(HBV-M)的相关性,探讨HBV-LP对反映体内HBV复制的意义。方法选取2013年4月至2015年9月深圳市龙岗区中医院收治的慢性乙型肝炎感染患者540例,采用实时荧光定量聚合酶链反应(PCR)检测患者的血清HBVDNA,采用酶联免疫吸附试验(ELISA)测定HBV-LP及HBV-M,对HBV-LP、HBV-M及HBV-DNA之间的相关性进行分析。结果乙型肝炎E抗原(HBeAg)阳性患者的HBV-LP阳性率为96.39%,而HBV-DNA的阳性率为93.33%,两者差异无统计学意义(P0.05);HBV-LP水平与HBV-DNA的拷贝数对数值呈正相关;而HBeAg阴性患者的HBV-LP阳性率为63.33%,HBV-DNA的阳性率为51.11%,两者差异有统计学意义(P0.05)。结论 HBV外膜大蛋白能够有效反映慢性乙型肝炎患者体内HBV复制情况,其灵敏度高于HBeAg,对于HBeAg阴性的慢性乙型肝炎患者而言,HBV-LP更能反映患者体内病毒的复制状态。
[Abstract]:Objective to analyze the serum immunological marker hepatitis B virus outer membrane macroprotein (HBV-LP) in patients with hepatitis B virus (HBV) infection. The correlation between HBV-DNA and HBV-M. To explore the significance of HBV-LP in reflecting the replication of HBV in vivo. Methods from April 2013 to September 2015, 540 patients with chronic hepatitis B infection were selected from Shenzhen Longgang District Hospital of traditional Chinese Medicine. The serum HBV DNA was detected by real-time fluorescence quantitative polymerase chain reaction (PCR), and HBV-LP and HBV-M were detected by enzyme linked immunosorbent assay (Elisa). The relationship between HBV-M and HBV-DNA was analyzed. Results the positive rate of HBV-LP was 96.39% in the patients with hepatitis B E antigen (HBeAg) positive. The positive rate of HBV-DNA was 93.33 and there was no significant difference between the two groups (P 0.05). The HBV-LP level was positively correlated with the copy number logarithm of HBV-DNA. The positive rate of HBV-LP in HBeAg negative patients was 63.33 and the positive rate of HBV-DNA was 51.11%. Conclusion the large outer membrane protein of HBV can effectively reflect the replication of HBV in patients with chronic hepatitis B, and its sensitivity is higher than that of HBeAg. HBV-LP can better reflect the replication status of HBeAg negative chronic hepatitis B patients.
【作者单位】：广东省深圳市龙岗区中医院检验科;广东省深圳市坪山新区人民医院检验科;广东省深圳市龙岗区中医院肝病科;
【基金】：广东省深圳市坪山新区卫生系统科研资助项目(201428)
【分类号】：R512.62;R440
【正文快照】： 慢性乙型肝炎是指由乙型肝炎病毒(HBV)引起的传染病。慢性乙型肝炎属于世界流行性疾病,报道称全球约有3.5亿人为HBV的携带者,而我国为HBV感染的高发国家[1]。近年来,随着乙型肝炎疫苗的普遍接种,HBV感染控制较为理想,但是关于抗HBV治疗效果的监测,包括体内病毒水平及复制情况,

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