福建省龙岩市2011年病毒性脑炎病例埃可病毒30型分析

发布时间：2018-03-11 11:41

  本文选题：病毒性脑炎　切入点：埃可病毒　出处：《中国疫苗和免疫》2015年05期 　论文类型：期刊论文

【摘要】：目的分析龙岩市2011年病毒性脑炎病例埃可病毒[人肠道致细胞病变孤儿病毒,Enteric Cytopathogenic Human Orphan(ECHO)Virus]30型(ECHO30型)特征。方法对龙岩市2011年某医院病毒性脑炎住院病例采集脑脊液(Cerebrospinal Fluid,CSF)进行病毒分离、血清中和试验定型;选取4株ECHO30型分离株,测定VP1编码区序列,与国内外其他病毒株进行比较。结果从142份患者CSF标本中分离到ECHO30型20株、ECHO11型7株、ECHO6型10株。4株ECHO30型VP1编码区序列长度为606个核苷酸,编码202个氨基酸。4株的核苷酸和氨基酸同源性分别为97%~100%和88%~100%。与2000年Bastianni原型株的核苷酸和氨基酸同源性分别为81%~82%和81%~93%。与2010年山东省、2011和2012年福州市流行毒株核苷酸和氨基酸同源性分别为96%~100%和87%~100%。系统进化树分析显示,4株龙岩分离株同属基因型(Genotype)Ⅱ。结论龙岩市2011年病毒性脑炎病例的ECHO_(30)型为GenotypeⅡ。
[Abstract]:Objective to analyze the characteristics of encephalitis virus type 30 (Enteric Cytopathogenic Human Orphan(ECHO)Virus) of 2011 viral encephalitis in Longyan city. Methods Cerebrospinal fluid (Cerebrospinal fluid) was collected from patients with viral encephalitis in a hospital in Longyan City on 2011. Fluido CSF) for virus isolation, Four isolates of ECHO30 type were selected and the sequence of VP1 coding region was determined. Results 20 strains of ECHO30 type 7 strains of ECHO6 type 7 strains of ECHO30 type 6 type 10 strains of ECHO30 type VP1 coding region length of 606 nucleotides were isolated from 142 patients with CSF. The nucleotide and amino acid homology of 202 amino acid 4 strains were 97 100% and 88 100%, respectively. The nucleotide and amino acid homology with 2000 Bastianni prototype strain were 81 2% and 81 0% respectively. The phylogenetic tree analysis showed that four Longyan isolates belong to Genotype.Conclusion in 2011, the virus encephalitis in Longyan City is Genotype 鈪，

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