贵阳市2012-2015年B型流行性感冒病毒血凝素基因特性分析

发布时间：2018-03-18 07:14

本文选题：流感病毒B型　切入点：血凝素类　出处：《中华疾病控制杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的了解2012-2015年贵阳市B型流行性感冒(以下简称流感)病毒的变异和流行特点,分析其与WHO推荐的疫苗株和我国的代表株匹配情况。方法将21份标本进行血凝素基因片段1(haemagglutinin 1,HA1)基因序列测定,通过DNA Star version 7.1等软件对测序产物进行分析。结果贵阳市B型流感病毒存在BY和BV两种谱系;核苷酸同源性显示,BV系与疫苗株B/Brisbane/60/2008的同源性为97.8%~99.3%;2013-2014年BY系与疫苗株B/Massachusetts/02/2012的同源性为96.2%~96.5%,与既往的疫苗株B/Wisconsin/01/2010同源性为99.0%~99.3%;2015年BY系与疫苗株B/PHUKET/3073/2013同源性为99.2%~99.5%;2012-2015年BV系与我国代表株B/Chongqing-Yuzhong/1384/2010同源性为96.8%~99.0%,BY系与种子疫苗株B/Hubei-Wujiagang/158/2009为98.2%~99.2%。与当年度疫苗株相比,2013-2014年BY系毒株有9个氨基酸位点发生变异,其中A134P位点涉及到抗原决定簇A区,N142K涉及到B区;2015年BY系毒株发生新的L198Q变异,且涉及到D区;BV系氨基酸位点替换多样,但很少涉及到抗原决定簇。结论 2012-2015年贵阳市人群中B型流感病毒在不断地发生改变,除2013-2014年疫苗株与流行株匹配性不佳以外,其余年度的匹配性均较好,能起到保护作用。
[Abstract]:Objective to investigate the variation and epidemic characteristics of influenza B virus in Guiyang from 2012 to 2015. To analyze the match between the vaccine strain recommended by WHO and the representative strain of our country, 21 samples were sequenced for haemagglutinin 1 haemagglutinin 1 (HA1) gene. The sequencing products were analyzed by DNA Star version 7.1. Results there were two lineages of by and BV of influenza B virus in Guiyang. The nucleotide homology between BV strain and vaccine strain B / Brisbane / 60 / 2008 was 97.8 / 99.3, and the homology between by strain and vaccine strain B / P / 02 / 2012 was 96.22.55.The homology with previous vaccine strain B / Wiscones / P / 01 / 2010 was 99.099.33.In 2015, the homology between by strain and vaccine strain BrPHUKET / 3073 / 2013 was 99.299.5% and 99.299.5% with our representative strain in 2012-2015. The homology of B / Chongqing-Yuzhong- / 1384 / 2010 is 96.880 / 99.0% and 98.22.It is 98.22.Compared with the vaccine strain of 2013-2014, there were nine amino acid loci in the virus strain of the by strain in 2013-2014, compared with the vaccine strain of the year 2013-2014, and the homology of by strain and seed vaccine strain B / Hubei-Wujiagangr / 158R / 2009 was 98.220.Compared with that of the current vaccine strain, The A134P locus was involved in the antigen determinant A region N142K and the B region. In 2015, a new L198Q mutation occurred in the by strain strain, and a variety of amino acid site substitution was involved in the D region BV strain. Conclusion the influenza B virus in Guiyang city from 2012 to 2015 is constantly changing. Except for the poor matching between vaccine strain and epidemic strain in 2013-2014, the matching between vaccine strain and epidemic strain in other years is better, which can play a protective role.
【作者单位】：贵州省疾病预防控制中心传染病防治研究所检验科;
【基金】：贵州省传染病预防与控制人才基地科研团队项目(RCJD1401)
【分类号】：R511.7

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