麻杏石甘汤与奥司他韦联合用药抗A型流感病毒效应研究

发布时间：2018-03-25 22:49

本文选题：麻杏石甘汤　切入点：奥司他韦　出处：《中华中医药杂志》2017年09期

【摘要】：目的:探讨麻杏石甘汤与奥司他韦联合用药抗A型流感病毒的实验效应。方法:(1)以血清药理学实验技术检测联合用药的含药血清体外对流感病毒感染MDCK细胞活性和流感病毒神经氨酸酶(NA)活性的影响。(2)以整体动物实验技术检测联合用药体内对流感病毒感染小鼠体质量、肺指数、肺组织病理变化的影响。结果:(1)各含药血清组MDCK细胞活性OD值较病毒对照组显著升高(P0.01),流感病毒NA活性较病毒对照组显著下降(P0.01)。联合用药组体外抗A型流感病毒效应显著优于麻杏石甘汤组(P0.01)。(2)各治疗组小鼠肺指数较病毒对照组显著降低(P0.01),肺组织病理变化损伤得到不同程度改善,其中联合用药组肺组织病理变化改善程度显著优于麻杏石甘汤组。结论:麻杏石甘汤与奥司他韦联合应用,具有较明显的协同增效作用。
[Abstract]:Objective: to investigate the experimental effect of Maxingshigan decoction combined with oseltamivir on influenza A virus. Methods: to detect the activity of combined drug containing serum against influenza virus infection MDCK cells in vitro by serum pharmacological experiment technique. And the effect of influenza virus neuraminidase (NAA) activity on the body mass of mice infected with influenza virus in vivo using the whole animal experiment technique. Lung index, Results the OD value of MDCK cell activity in each drug serum group was significantly higher than that of virus control group, and the na activity of influenza virus was significantly lower than that of virus control group. The lung index of each treatment group was significantly lower than that of virus control group, and the pathological changes of lung tissue were improved in different degree. Conclusion: the combination of Maxingshigan decoction and oseltamivir has obvious synergistic effect.
【作者单位】：湖南中医药大学第一附属医院;湖南中医药大学中医学院;湖南中医药大学医学院;
【基金】：国家自然科学基金项目(No.81473468,No.81503445) 湖南省自然科学基金项目(No.13JJ6062,No.2016JJ2095) 湖南省教育厅项目(No.17K067,No.13A066,No.15C1032) 湖南省卫生计生委科研课题(No.C2015-16,No.C2016049) 湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目(No.15) 湖南省研究生创新性实验项目(No.CX2015B325) 湖南中医药大学重点学科《基础医学》、《病原生物学》资助项目(No.1)~~
【分类号】：R511.7

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