2015-2016年广东省珠海市风疹病毒分子流行病学研究

发布时间：2018-04-04 23:36

本文选题：风疹病毒　切入点：基因型　出处：《中国疫苗和免疫》2017年01期

【摘要】：目的分析珠海市2015-2016年风疹病毒基因型别和特征,为风疹控制和消除提供分子流行病学基线数据。方法采集2015-2016年珠海市疑似风疹病例出疹后3天内的咽拭子,使用荧光逆转录-聚合酶链反应(RT-PCR)方法检测风疹病毒核酸,对阳性标本进行风疹病毒E1基因739个核苷酸片段(nt8731-nt9469)的扩增、序列测定和分析,与32株13个基因型的WHO参考株、中国其他省份流行的2B基因型分别构建基因亲缘性关系树。结果2015-2016年共收集疑似风疹病例的42份咽拭子标本,其中23份病毒核酸检测阳性,经进一步扩增,18份标本获得了靶基因序列。基因亲缘性关系分析显示,18份标本均为2B基因型风疹病毒,核苷酸和氨基酸序列同源性分别为98.2%-100%和99.1%-100%,并可分成两个进化分支,不同的进化分支包含了其他省份2B基因型分离株,无地理局限性和年代相关性。18株2B基因型风疹病毒在N-型糖基化位点、抗原表位、中和抗原决定簇和血凝抑制位点没有发生突变。结论 2015-2016年珠海市流行2B基因型风疹病毒,与全国流行趋势一致。应继续加强珠海市风疹病毒分子流行病学监测。
[Abstract]:Objective to analyze the gene types and characteristics of rubella virus from 2015 to 2016 in Zhuhai, and to provide molecular epidemiological baseline data for the control and elimination of rubella.Methods pharyngeal swabs of suspected rubella cases from 2015 to 2016 in Zhuhai were collected within 3 days after rash. The nucleic acid of rubella virus was detected by fluorescence reverse transcriptase-polymerase chain reaction (RT-PCR).The 739 nucleotide fragments of rubella virus E1 gene were amplified, sequenced and analyzed. The genetic relationship tree was constructed with 32 WHO reference strains of 13 genotypes and 2B genotypes prevalent in other provinces of China.Results A total of 42 pharyngeal swabs of suspected rubella cases were collected from 2015 to 2016, 23 of which were positive for nucleic acid detection, and 18 samples were further amplified to obtain the target gene sequence.Genetic analysis showed that all 18 samples were rubella virus of 2B genotype, the homology of nucleotide and amino acid sequences were 98.2-100% and 99.1-100%, respectively, and could be divided into two evolutionary branches.There were no mutations in N-type glycosylation sites, antigen epitopes, neutralizing antigenic determinants and hemagglutination inhibition sites of rubella virus with no geographical limitation and age correlation.Conclusion the prevalence of rubella virus genotype 2B in Zhuhai from 2015 to 2016 is consistent with the trend of epidemic in China.Molecular epidemiological surveillance of rubella virus should continue to be strengthened in Zhuhai City.
【作者单位】：珠海市疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所;
【分类号】：R511.2

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